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Literature summary for 2.8.2.2 extracted from
- Studies on a 3beta-hydroxysteroid sulphotransferase from rat liver (1976), Biochim. Biophys. Acta, 429, 391-401.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| glutathione | activity of the purified enzyme is slightly enhanced | Rattus norvegicus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| adenosine 3',5'-bisphosphate | enzyme appears to be highly suspectible to product inhibition | Rattus norvegicus | |
| HgCl2 | - |
Rattus norvegicus | |
| additional information | EDTA has no effect | Rattus norvegicus | |
| p-chloromercuribenzoate | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.006 | - |
dehydroepiandrosterone | pH 8.0, 37°C | Rattus norvegicus | |
| 0.013 | - |
3'-phosphoadenylylsulfate | pH 8.0, 37°C | Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | crude enzyme activity is markedly stimulated by divalent cations like Fe2+, Co2+ and Mn2+, this effect is not observed with purified enzyme | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylylsulfate + dehydroepiandrosterone | Rattus norvegicus | - |
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
rat | - |
| Rattus norvegicus | - |
Wistar, female | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.01716 | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, activity towards dehydroepiandrosterone as acceptor substrate is stable for several months | Rattus norvegicus |
| 4°C, activity declines by 60% after 4 weeks | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylylsulfate + 1-butanol | relative activity 7% | Rattus norvegicus | adenosine 3',5'-bisphosphate + 1-butanoyl sulfate | - |
r | |
| 3'-phosphoadenylylsulfate + 11-deoxycorticosterone | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + 11-deoxycorticosterone sulfate | - |
r | |
| 3'-phosphoadenylylsulfate + 11-deoxycorticosterone | relative activity 10% | Rattus norvegicus | adenosine 3',5'-bisphosphate + 11-deoxycorticosterone sulfate | - |
r | |
| 3'-phosphoadenylylsulfate + 3alpha-hydroxy-5beta-androstan-17-one | relative activity 5% | Rattus norvegicus | adenosine 3',5'-bisphosphate + 5beta-androstan-17-one 3-sulfate | - |
? | |
| 3'-phosphoadenylylsulfate + 3beta,17alpha-dihydroxy-pregn-5-ene-20-one | relative activity 4% | Rattus norvegicus | adenosine 3',5'-bisphosphate + ? | - |
r | |
| 3'-phosphoadenylylsulfate + 3beta-hydroxy-5beta-androstan-17-one | relative activity 45% | Rattus norvegicus | adenosine 3',5'-bisphosphate + 5beta-androstan-17-one 3-sulfate | - |
r | |
| 3'-phosphoadenylylsulfate + 5alpha-androstane-3alpha,17beta-diol | relative activity 20% | Rattus norvegicus | adenosine 3',5'-bisphosphate + ? | - |
r | |
| 3'-phosphoadenylylsulfate + 5alpha-androstane-3beta,17beta-diol | relative activity 83% | Rattus norvegicus | adenosine 3',5'-bisphosphate + ? | - |
r | |
| 3'-phosphoadenylylsulfate + 5alpha-pregnane-3beta,20alpha-diol | relative activity 86% | Rattus norvegicus | adenosine 3',5'-bisphosphate + ? | - |
r | |
| 3'-phosphoadenylylsulfate + 5beta-androstane-3alpha,17beta-diol | relative activity 7% | Rattus norvegicus | adenosine 3',5'-bisphosphate + ? | - |
r | |
| 3'-phosphoadenylylsulfate + alpha-naphthol | relative activity 4% | Rattus norvegicus | adenosine 3',5'-bisphosphate + alpha-naphthyl sulfate | - |
r | |
| 3'-phosphoadenylylsulfate + androst-5-en-3beta,17beta-diol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + androst-5-en-3beta,17beta-diol sulfate | - |
? | |
| 3'-phosphoadenylylsulfate + androsterone | 3alpha-hydroxy-5alpha-androstan-17-one | Rattus norvegicus | adenosine 3',5'-bisphosphate + 5alpha-androstan-17-one 3-sulfate | - |
r | |
| 3'-phosphoadenylylsulfate + androsterone | relative activity 53% | Rattus norvegicus | adenosine 3',5'-bisphosphate + 5alpha-androstan-17-one 3-sulfate | - |
r | |
| 3'-phosphoadenylylsulfate + corticosterone | relative activity 6% | Rattus norvegicus | adenosine 3',5'-bisphosphate + corticosterone sulfate | - |
r | |
| 3'-phosphoadenylylsulfate + dehydroepiandrosterone | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
r | |
| 3'-phosphoadenylylsulfate + epiandrosterone | 3beta-hydroxy-5alpha-androstan-17-one | Rattus norvegicus | adenosine 3',5'-bisphosphate + 5alpha-androstan-17-one 3-sulfate | - |
r | |
| 3'-phosphoadenylylsulfate + L-tyrosinemethyl ester | relative activity 9% | Rattus norvegicus | adenosine 3',5'-bisphosphate + ? | - |
r | |
| 3'-phosphoadenylylsulfate + pregnenolone | relative activity 61% | Rattus norvegicus | adenosine 3',5'-bisphosphate + 5-pregnen-20-one 3-sulfate | - |
r | |
| 3'-phosphoadenylylsulfate + pregnenolone | 3beta-hydroxy-5-pregnen-20-one | Rattus norvegicus | adenosine 3',5'-bisphosphate + 5-pregnen-20-one 3-sulfate | - |
r | |
| 3'-phosphoadenylylsulfate + testosterone | relative activity 2% | Rattus norvegicus | adenosine 3',5'-bisphosphate + testosterone 3-sulfate | - |
r | |
| additional information | inactive towards estrogens, cholesterol and ergocalciferol, maximum activity is observed when the steroid is of the allo-series and possesses a 3beta-hydroxyl and 17-oxo function, estrone, estradiol, cortisol, 11beta-hydroxy-pregn-4-ene-3-20-dione, beta-naphthol, alpha-naphthylamine, bilirubin and diethylstilbestrol are no substrates for the purified enzyme | Rattus norvegicus | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | - |
Rattus norvegicus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
unstable at low pH | Rattus norvegicus |
| 6 | 9 | stable for 30 min at 37°C | Rattus norvegicus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
adenosine 3',5'-bisphosphate | pH 8.0, 37°C | Rattus norvegicus | |
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