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Literature summary for 2.8.2.15 extracted from

  • Ekuase, E.J.; Liu, Y.; Lehmler, H.J.; Robertson, L.W.; Duffel, M.W.
    Structure-activity relationships for hydroxylated polychlorinated biphenyls as inhibitors of the sulfation of dehydroepiandrosterone catalyzed by human hydroxysteroid sulfotransferase SULT2A1 (2011), Chem. Res. Toxicol., 24, 1720-1728.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.2.15

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
3',4'-dihydroxy-2,3-dichlorobiphenyl
-
 Homo sapiens
3',4'-dihydroxy-4-monochlorobiphenyl
-
 Homo sapiens
4'-hydroxy-2',3,4-trichlorobiphenyl
-
 Homo sapiens
4'-hydroxy-2,3',4,5'-tetrachlorobiphenyl
-
 Homo sapiens
4'-hydroxy-2,3',4-trichlorobiphenyl
-
 Homo sapiens
4'-hydroxy-2,3'-dichlorobiphenyl
-
 Homo sapiens
4'-hydroxy-2,5-dichlorobiphenyl
-
 Homo sapiens
4'-hydroxy-3,4-dichlorobiphenyl
-
 Homo sapiens
4'-hydroxy-4-monochlorobiphenyl
-
 Homo sapiens
4-hydroxy-2',3,5-trichlorobiphenyl
-
 Homo sapiens
4-hydroxy-2,4'-dichlorobiphenyl
-
 Homo sapiens
4-hydroxy-3,3',5-trichlorobiphenyl
-
 Homo sapiens
4-hydroxy-3,3'-dichlorobiphenyl
-
 Homo sapiens
4-hydroxy-3,5-dichlorobiphenyl
-
 Homo sapiens
6'-hydroxy-3,3'-4-trichlorobiphenyl
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0008
-
 dehydroepiandrosterone in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C Homo sapiens
0.0081
-
 4-hydroxy-2',3,5-trichlorobiphenyl in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C Homo sapiens
0.0085
-
 4-hydroxy-3,5-dichlorobiphenyl in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C Homo sapiens
0.0086
-
 4'-hydroxy-2,3',4-trichlorobiphenyl in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C Homo sapiens
0.0136
-
 4-hydroxy-3,3',5-trichlorobiphenyl in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C Homo sapiens
0.0262
-
 4'-hydroxy-2',3,4-trichlorobiphenyl in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C Homo sapiens
0.0366
-
 4-hydroxy-3,3'-dichlorobiphenyl in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C Homo sapiens
0.0568
-
 4-hydroxy-2,4'-dichlorobiphenyl in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33785
-
 x * 33785, calculated from amino acid sequence Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylylsulfate + 4'-hydroxy-2',3,4-trichlorobiphenyl Homo sapiens
-
 adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + 4'-hydroxy-2,3',4-trichlorobiphenyl Homo sapiens
-
 adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + 4-hydroxy-2',3,5-trichlorobiphenyl Homo sapiens
-
 adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + 4-hydroxy-2,4'-dichlorobiphenyl Homo sapiens
-
 adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + 4-hydroxy-3,3',5-trichlorobiphenyl Homo sapiens
-
 adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + 4-hydroxy-3,3'-dichlorobiphenyl Homo sapiens
-
 adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + 4-hydroxy-3,5-dichlorobiphenyl Homo sapiens
-
 adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + dehydroepiandrosterone Homo sapiens
-
 adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylylsulfate + 4'-hydroxy-2',3,4-trichlorobiphenyl
-
 Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + 4'-hydroxy-2,3',4-trichlorobiphenyl
-
 Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + 4-hydroxy-2',3,5-trichlorobiphenyl
-
 Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + 4-hydroxy-2,4'-dichlorobiphenyl
-
 Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + 4-hydroxy-3,3',5-trichlorobiphenyl
-
 Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + 4-hydroxy-3,3'-dichlorobiphenyl
-
 Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + 4-hydroxy-3,5-dichlorobiphenyl
-
 Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + dehydroepiandrosterone
-
 Homo sapiens adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
 ?

Subunits

Subunits Comment Organism
? x * 33785, calculated from amino acid sequence Homo sapiens

Synonyms

Synonyms Comment Organism
hydroxysteroid sulfotransferase
-
 Homo sapiens
SULT2A1
-
 Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0006
-
 in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C Homo sapiens 4-hydroxy-2',3,5-trichlorobiphenyl
0.0008
-
 in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C Homo sapiens 4'-hydroxy-2,3',4,5'-tetrachlorobiphenyl
0.0025
-
 in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C Homo sapiens 4'-hydroxy-2',3,4-trichlorobiphenyl
0.0044
-
 in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C Homo sapiens 4'-hydroxy-2,3',4-trichlorobiphenyl
0.0044
-
 in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C Homo sapiens 4-hydroxy-3,3',5-trichlorobiphenyl
0.0075
-
 in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C Homo sapiens 4'-hydroxy-2,5-dichlorobiphenyl
0.0098
-
 in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C Homo sapiens 4'-hydroxy-2,3'-dichlorobiphenyl
0.01
-
 in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C Homo sapiens 4-hydroxy-2,4'-dichlorobiphenyl
0.013
-
 in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C Homo sapiens 4-hydroxy-3,5-dichlorobiphenyl
0.014
-
 in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C Homo sapiens 4-hydroxy-3,3'-dichlorobiphenyl
0.016
-
 in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C Homo sapiens 4'-hydroxy-3,4-dichlorobiphenyl
0.016
-
 in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C Homo sapiens 6'-hydroxy-3,3'-4-trichlorobiphenyl
0.016
-
 in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C Homo sapiens 3',4'-dihydroxy-2,3-dichlorobiphenyl
0.028
-
 in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C Homo sapiens 4'-hydroxy-4-monochlorobiphenyl
0.096
-
 in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C Homo sapiens 3',4'-dihydroxy-4-monochlorobiphenyl