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Literature summary for 2.8.2.15 extracted from
- Characterization of proline-serine-rich carboxyl terminus in human sulfotransferase 2B1b: immunogenicity, subcellular localization, kinetic properties, and phosphorylation (2006), Drug Metab. Dispos., 34, 1749-1755.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged full-length SULT2B1b and truncated SULT2B1b without the PSC extension in Escherichia coli strain DH5alpha and in human BeWo choriocarcinoma cells, translocation of the wild-type enzyme to nuclei, but not of the truncation mutant | Homo sapiens |
| full-length isoform SULT2B1b as well as truncated SULT2B1b without the PS proline- and serine-rich carboxyl extension are expressed in Escherichia coli XL1-Blue cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of a SULT2B1b truncation mutant lacking the proline- and serine-rich carboxyl end of about 53 amino acids, removal of the PSC extension significantly decreases the thermostability of the expressed enzyme and the rate of dehydroepiandrosterone sulfation, truncated SULT2B1b is incapable of translocation to nuclei in transfected human BeWo choriocarcinoma cells | Homo sapiens |
General Stability
| General Stability | Organism |
|---|---|
| removal of the extension at the proline- and serine-rich carboxyl end of about 53 amino acids significantly decreases the thermostability of the expressed enzyme as well as decreasing the rate of dehydroepiandrosterone sulfation | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | the proline- and serine-rich carboxyl end is an important site in the immunogenicity, nuclear translocation, kinetic activity, and thermostability of SULT2B1b | Homo sapiens | |
| 0.0006 | - |
dehydroepiandrosterone | truncated isoform SULT2B1b, in 50 mM Tris-HCl, pH 7.5, 10 mM MgCl2, at 37°C | Homo sapiens |  |
| 0.0038 | - |
dehydroepiandrosterone | full-length isoform SULT2B1b, in 50 mM Tris-HCl, pH 7.5, 10 mM MgCl2, at 37°C | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Homo sapiens | 5829 | - |
| cytosol | truncated mutant SULT2B1b lacking the proline- and serine-rich carboxyl end | Homo sapiens | 5829 | - |
| microsome | - |
Homo sapiens | - |
- |
| nucleus | - |
Homo sapiens | 5634 | - |
| nucleus | full-length SULT2B1b, the proline- and serine-rich carboxyl end is an important site in the immunogenicity, nuclear translocation, kinetic activity, and thermostability of SULT2B1b | Homo sapiens | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylyl sulfate + dehydroepiandrosterone | Homo sapiens | - |
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-O-sulfate | - |
? | |
| 3'-phosphoadenylylsulfate + dehydroepiandrosterone | Homo sapiens | - |
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Homo sapiens | - |
two isozymes SULT2B1a and SULT2B1b | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | the the proline- and serine-rich carboxyl end of SULT2B1 is phosphorylated at serine residues, e.g. by casein kinase or Cdc2 protein kinase in vitro | Homo sapiens |
| phosphoprotein | the full-length enzyme is phosphorylated by casein kinase or Cdc2 protein kinase in vitro | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography and Protein A-Sepharose column chromatography | Homo sapiens |
| recombinant His-tagged full-length SULT2B1b as well as truncated SULT2B1b without the PSC extension from Escherichia coli strain DH5alpha by nickel affinity chromatography | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylyl sulfate + dehydroepiandrosterone | - |
Homo sapiens | adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-O-sulfate | - |
? | |
| 3'-phosphoadenylylsulfate + dehydroepiandrosterone | - |
Homo sapiens | adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | isozyme SULT2B1b has an extension at the proline- and serine-rich carboxyl end of about 53 amino acids | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| sulfotransferase 2B1 | - |
Homo sapiens |
| sulfotransferase 2B1b | - |
Homo sapiens |
| SULT2B1 | - |
Homo sapiens |
| SULT2B1b | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the proline- and serine-rich carboxyl end is an important site in the immunogenicity, nuclear translocation, kinetic activity, and thermostability of SULT2B1b | Homo sapiens |
| 42 | - |
after 30 min, dehydroepiandrosterone sulfation activity with recombinant truncated SULT2B1b is decreased 3.5fold more than that of recombinant full-length SULT2B1b, after 45 min,the truncated SULT2B1b activity is completely eliminated, whereas full-length SULT2B1b still maintains about 70% of its original activity | Homo sapiens |
| 42 | 45 | truncated SULT2B1b is more sensitive to thermal inactivation than the full-length enzyme. After incubation at 42°C for 30 min, dehydroepiandrosterone sulfation activity with truncated SULT2B1b is decreased 3.5fold more than that of full-length enzyme. After 45 min of incubation at 42°C, truncated SULT2B1b activity is completely eliminated, whereas full-length SULT2B1b still maintains about 70% of its original activity | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
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