Literature summary for 2.8.2.15 extracted from

Liu, Y.; Apak, T.I.; Lehmler, H.J.; Robertson, L.W.; Duffel, M.W.
Hydroxylated polychlorinated biphenyls are substrates and inhibitors of human hydroxysteroid sulfotransferase SULT2A1 (2006), Chem. Res. Toxicol., 19, 1420-1425.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression of SULT1A2 in Escherichia coli strain BL21 (DE3)	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
4'-hydroxy-2,5-dichlorobiphenyl	i.e. 4'-OH PCB 9, inhibition of SULT1A2	Homo sapiens
4-hydroxy-2',3,5-trichlorobiphenyl	i.e. 4-OH PCB 34, substrate inhibition of SULT1A2	Homo sapiens
dehydroepiandrosterone	i.e. DHEA, substrate inhibition of SULT1A2	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	for 4'-OH PCB 68, the limit of solubility makes the determination of kinetic constants difficult, the highest rate of sulfation is observed at the limit of solubility at about 0.05 mM	Homo sapiens
0.025	-	dehydroepiandrosterone	pH 7.0, 37°C, recombinant SULT1A2	Homo sapiens
0.046	-	4-hydroxy-2',3,5-trichlorobiphenyl	pH 7.0, 37°C, recombinant SULT1A2	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3'-phosphoadenylyl sulfate + dehydroepiandrosterone	Homo sapiens	-	adenosine 3',5'-bisphosphate + dehydroepiandrosterone O-sulfate	-	?
additional information	Homo sapiens	hydroxylated polychlorinated biphenyls may compete with other xenobiotic substrates as well as endogenous substrates for SULT2A1	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	SULT2A1	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0175	-	purified recombinant SULT1A2, substrate 4'-hydroxy-2,3',4,5'-tetrachlorobiphenyl	Homo sapiens
0.027	-	purified recombinant SULT1A2, substrate 4-hydroxy-2',3,5-trichlorobiphenyl	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3'-phosphoadenylyl sulfate + 4'-hydroxy-2,3',4,5'-tetrachlorobiphenyl	i.e. 4'-OH PCB 68, substrate of SULT1A2	Homo sapiens	adenosine 3',5'-bisphosphate + 4'-hydroxy-2,3',4,5'-tetrachlorobiphenyl 4'-O-sulfate	-	?
3'-phosphoadenylyl sulfate + 4-hydroxy-2',3,5-trichlorobiphenyl	i.e. 4-OH PCB 34, substrate of SULT1A2	Homo sapiens	adenosine 3',5'-bisphosphate + 2',3,5-trichlorobiphenyl-4-yl hydrogen sulfate	-	?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone	i.e. DHEA, substrate of SULT1A2	Homo sapiens	adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-O-sulfate	-	?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone	-	Homo sapiens	adenosine 3',5'-bisphosphate + dehydroepiandrosterone O-sulfate	-	?
additional information	hydroxylated polychlorinated biphenyls may compete with other xenobiotic substrates as well as endogenous substrates for SULT2A1	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
DHEA-ST	-	Homo sapiens
hydroxysteroid sulfotransferase	-	Homo sapiens
More	SULT2A1 belongs to the family of phenol sulfotransferases	Homo sapiens
ST2A3	-	Homo sapiens
SULT2A1	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.112	-	dehydroepiandrosterone	pH 7.0, 37°C, recombinant SULT1A2	Homo sapiens
0.253	-	4-hydroxy-2',3,5-trichlorobiphenyl	pH 7.0, 37°C, recombinant SULT1A2	Homo sapiens

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.0039	-	pH 7.0, 37°C, recombinant SULT1A2	Homo sapiens	4'-hydroxy-2,5-dichlorobiphenyl

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Release 2023.1 (January 2023)