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Literature summary for 2.8.2.15 extracted from
- Expression and characterization of the human 3b-hydroxysteroid sulfotransferases (SULT2B1alpha and SULT2B1beta) (2001), J. Steroid Biochem. Mol. Biol., 77, 261-269.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Homo sapiens |
| SULT2B1 gene encodes 2 isoforms, SULT2B1a and SULT2B1b, generated by alternate splicing of the first exon, cloned and expressed in Escherichia coli | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0003 | - |
3'-phosphoadenylylsulfate | pH 7.4, 37°C, isoenzyme His-SULT2B1a | Homo sapiens |  |
| 0.0006 | - |
3'-phosphoadenylylsulfate | pH 7.4, 37°C, isoenzyme His-SULT2B1b | Homo sapiens | |
| 0.0008 | - |
3'-phosphoadenylylsulfate | pH 7.4, 37°C, isoenzyme DHEA-ST | Homo sapiens | |
| 0.0018 | - |
pregnenolone | pH 7.4, 37°C, isoenzyme His-SULT2B1b | Homo sapiens | |
| 0.0019 | - |
3beta-hydroxyandrost-5-ene-17-one | pH 7.4, 37°C, isoenzyme DHEA-ST | Homo sapiens | |
| 0.0027 | - |
pregnenolone | pH 7.4, 37°C, isoenzyme SULT2B1b | Homo sapiens | |
| 0.003 | - |
pregnenolone | pH 7.4, 37°C, isoenzyme His-SULT2B1a | Homo sapiens | |
| 0.0044 | - |
3beta-hydroxyandrost-5-ene-17-one | pH 7.4, 37°C, isoenzyme His-SULT2B1a | Homo sapiens | |
| 0.0049 | - |
pregnenolone | pH 7.4, 37°C, isoenzyme DHEA-ST | Homo sapiens | |
| 0.0072 | - |
3'-phosphoadenylylsulfate | pH 7.4, 37°C, isoenzyme SULT2B1a | Homo sapiens | |
| 0.0072 | - |
pregnenolone | pH 7.4, 37°C, isoenzyme SULT2B1a | Homo sapiens | |
| 0.0093 | - |
3beta-hydroxyandrost-5-ene-17-one | pH 7.4, 37°C, isoenzyme SULT2B1a | Homo sapiens | |
| 0.0096 | - |
3'-phosphoadenylylsulfate | pH 7.4, 37°C, isoenzyme SULT2B1b | Homo sapiens | |
| 0.0109 | - |
3beta-hydroxyandrost-5-ene-17-one | pH 7.4, 37°C, isoenzyme His-SULT2B1b | Homo sapiens | |
| 0.0185 | - |
3beta-hydroxyandrost-5-ene-17-one | pH 7.4, 37°C, isoenzyme SULT2B1b | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Homo sapiens | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39620 | - |
isoform SULT2B1a | Homo sapiens |
| 41300 | - |
isoform SULT2B1b | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylylsulfate + dehydroepiandrosterone | Homo sapiens | isoform SULT2B1 is capable of regulating the activity of adrenal androgens via their inactivation by sulfation | adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
human | - |
| Homo sapiens | O00204 | human, isoenzyme SULT2B1a | - |
| Homo sapiens | O00204 | human, isoenzyme SULT2B1b | - |
| Homo sapiens | Q06520 | human, isoenzyme DHEA-ST | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| SULT2B1 | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adrenal cortex | - |
Homo sapiens | - |
| liver | - |
Homo sapiens | - |
| additional information | no SULT2B1 in liver, colon, lung, kidney, brain, or testis | Homo sapiens | - |
| placenta | SULT2B1 | Homo sapiens | - |
| prostate | SULT2B1 | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylylsulfate + 3beta-hydroxy-5alpha-androstan-17-one | SULT2B1 isoform | Homo sapiens | adenosine 3',5'-bisphosphate + 5alpha-androstan-17-one 3-sulfate | - |
r | |
| 3'-phosphoadenylylsulfate + androstenediol | SULT2B1 isoform | Homo sapiens | adenosine 3',5'-bisphosphate + ? | - |
r | |
| 3'-phosphoadenylylsulfate + dehydroepiandrosterone | SULT2B1 isoform | Homo sapiens | adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
r | |
| 3'-phosphoadenylylsulfate + dehydroepiandrosterone | isoform SULT2B1 is capable of regulating the activity of adrenal androgens via their inactivation by sulfation | Homo sapiens | adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
r | |
| 3'-phosphoadenylylsulfate + genistein | - |
Homo sapiens | adenosine 3',5'-bisphosphate + ? | - |
r | |
| 3'-phosphoadenylylsulfate + pregnenolone | SULT2B1 isoform | Homo sapiens | adenosine 3',5'-bisphosphate + pregnenolone sulfate | - |
r | |
| additional information | isoform SULT2B1, no activity detected towards testosterone, dihydrotestosterone, dexamethasone, beta-estradiol, cholesterol, cortisol, androsterone, or p-nitrophenol, no antibody reaction with EST, P-PST-1, M-PST, DHEA-ST, or ST1B2 during immunoblot analysis | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DHEA-ST | - |
Homo sapiens |
| SULT2B1 | - |
Homo sapiens |
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