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Literature summary for 2.8.2.14 extracted from
- Enzymic sulfation of bile salts. Partial purification and characterization of an enzyme from the liver of the shark Heterodontus portusjacksoni that catalyzes the sulfation of the shark bile steroid 5beta-scymnol (1994), Comp. Biochem. Physiol. B, 107, 461-469.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetate | - |
Heterodontus portusjacksoni |  |
| p-chloromercuribenzoate | - |
Heterodontus portusjacksoni | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
3'-phosphoadenylylsulfate | - |
Heterodontus portusjacksoni | |
| 0.014 | - |
5beta-scymnol | - |
Heterodontus portusjacksoni | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Heterodontus portusjacksoni | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
gel filtration | Heterodontus portusjacksoni |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Heterodontus portusjacksoni | - |
shark | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially | Heterodontus portusjacksoni |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Heterodontus portusjacksoni | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylyl-sulfate + 5beta-scymnol | bile steroid | Heterodontus portusjacksoni | adenosine 3',5'-bisphosphate + 5beta-scymnol sulfate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Heterodontus portusjacksoni |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | little activity recorded at pH 5.0 and pH 8.0 | Heterodontus portusjacksoni |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Heterodontus portusjacksoni | isoelectric focusing, 2 major bands with different pI values | - |
5 |
| Heterodontus portusjacksoni | isoelectric focusing, 2 major bands with different pI values | 6.69 | 5.85 |
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