General Stability | Organism |
---|---|
freezing and thawing results in significant loss in activity, substantial stabilization with addition of sucrose | Cavia porcellus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
7,12-diketochenodeoxycholate | - |
Cavia porcellus | |
adenosine-3',5'-diphosphate | - |
Cavia porcellus | |
ATP | - |
Cavia porcellus | |
EDTA | - |
Cavia porcellus | |
iodoacetate | - |
Cavia porcellus | |
NaF | - |
Cavia porcellus | |
NaN3 | - |
Cavia porcellus | |
p-chloromercuribenzoate | - |
Cavia porcellus | |
Taurodehydrocholate | - |
Cavia porcellus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0014 | - |
3'-phosphoadenylylsulfate | pH 6.5, 37°C | Cavia porcellus | |
0.077 | - |
taurolithocholate | pH 6.5, 37°C | Cavia porcellus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | activity 107% | Cavia porcellus | |
CuCl2 | activity 136% | Cavia porcellus | |
CuSO4 | activity 107% | Cavia porcellus | |
FeSO4 | activity 134% | Cavia porcellus | |
KCl | activity 128% | Cavia porcellus | |
LiCl | activity 108% | Cavia porcellus | |
Mg2+ | required for activity | Cavia porcellus | |
ZnSO4 | activity 111% | Cavia porcellus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
76000 | - |
gel filtration | Cavia porcellus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + taurolithocholate | Cavia porcellus | - |
adenosine 3',5'-bisphosphate + taurolithocholate sulfate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cavia porcellus | - |
guinea pig | - |
Purification (Comment) | Organism |
---|---|
- |
Cavia porcellus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Cavia porcellus | - |
additional information | no activity in kidney | Cavia porcellus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0216 | - |
- |
Cavia porcellus |
Storage Stability | Organism |
---|---|
-20°C, (NH4)2SO4 precipitate, stable for several months | Cavia porcellus |
4°C, loses about 50% activity in 1 week | Cavia porcellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + isolithocholic acid | 5beta-cholanoic acid-3beta-ol | Cavia porcellus | adenosine 3',5'-bisphosphate + isolithocholate sulfate | - |
? | |
3'-phosphoadenylyl sulfate + taurolithocholate | - |
Cavia porcellus | adenosine 3',5'-bisphosphate + taurolithocholate sulfate | - |
? | |
3'-phosphoadenylyl-sulfate + 5-cholenic acid-3beta-ol | - |
Cavia porcellus | adenosine 3',5'-bisphosphate + 5-cholenic acid-3-beta-ol sulfate | - |
? | |
3'-phosphoadenylyl-sulfate + 5alpha-cholanoic acid-3beta-ol | - |
Cavia porcellus | adenosine 3',5'-bisphosphate + 5alpha-cholanic acid-3beta-ol sulfate | - |
? | |
3'-phosphoadenylyl-sulfate + glycochenodeoxycholate | - |
Cavia porcellus | adenosine 3',5'-bisphosphate + glycochenodeoxycholate monosulfate | - |
? | |
3'-phosphoadenylyl-sulfate + glycocholate | - |
Cavia porcellus | adenosine 3',5'-bisphosphate + glycocholate 3-sulfate | - |
? | |
3'-phosphoadenylyl-sulfate + glycolithocholate | - |
Cavia porcellus | adenosine 3',5'-bisphosphate + glycolithocholate 3-sulfate | - |
? | |
3'-phosphoadenylyl-sulfate + lithocholate | - |
Cavia porcellus | adenosine 3',5'-bisphosphate + lithocholate 3-sulfate | - |
? | |
3'-phosphoadenylyl-sulfate + taurochenodeoxycholate | - |
Cavia porcellus | adenosine 3',5'-bisphosphate + taurochenodeoxycholate monosulfate + taurochenodeoxycholate disulfate | - |
? | |
3'-phosphoadenylyl-sulfate + taurocholate | - |
Cavia porcellus | adenosine 3',5'-bisphosphate + taurocholate monosulfate + taurocholate disulfate + taurocholate trisulfate | - |
? | |
additional information | purified enzyme does not react with estrone, estradiol, testosterone, dehydroepiandrosterone, cholesterol, phenol, tyramine, and serotonin | Cavia porcellus | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
- |
Cavia porcellus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Cavia porcellus | - |
5.9 | 5.6 |