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Literature summary for 2.8.2.14 extracted from
- Enzymatic sulfation of bile salts. II. Studies on bile salt sulfotranferase from rat kidney (1978), Biochim. Biophys. Acta, 522, 443-451.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| adenosine-3',5'-diphosphate | - |
Rattus norvegicus |  |
| ATP | - |
Rattus norvegicus | |
| EDTA | - |
Rattus norvegicus | |
| iodoacetate | - |
Rattus norvegicus | |
| isolithocholate | - |
Rattus norvegicus | |
| additional information | no inhibition is observed with 0.1 mM phenol | Rattus norvegicus | |
| NaF | - |
Rattus norvegicus | |
| NaN3 | - |
Rattus norvegicus | |
| p-chloromercuribenzoate | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
3'-phosphoadenylylsulfate | pH 6.5, 37°C | Rattus norvegicus | |
| 0.02 | - |
glycolithocholate | pH 6.5, 37°C | Rattus norvegicus | |
| 0.02 | - |
taurolithocholate | pH 6.5, 37°C | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
kidney enzyme, gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylyl sulfate + taurolithocholate | Rattus norvegicus | sulfation of hepatotoxic monohydroxy bile salts as important detoxification mechanism to avoid hepatic damages by the substrates, protective mechanism against accumulation of hepatotoxic bile salts | adenosine 3',5'-bisphosphate + taurolithocholate sulfate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
rat | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0049 | - |
kidney enzyme | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylyl sulfate + taurolithocholate | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + taurolithocholate sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + taurolithocholate | sulfation of hepatotoxic monohydroxy bile salts as important detoxification mechanism to avoid hepatic damages by the substrates, protective mechanism against accumulation of hepatotoxic bile salts | Rattus norvegicus | adenosine 3',5'-bisphosphate + taurolithocholate sulfate | - |
? | |
| 3'-phosphoadenylyl-sulfate + chenodeoxycholate | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + chenodeoxycholate 7-sulfate | - |
? | |
| 3'-phosphoadenylyl-sulfate + glycochenodeoxycholate | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + glycochenodeoxycholate monosulfate | - |
? | |
| 3'-phosphoadenylyl-sulfate + glycocholate | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + glycocholate 3-sulfate | - |
? | |
| 3'-phosphoadenylyl-sulfate + glycolithocholate | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + glycolithocholate 3-sulfate | - |
? | |
| 3'-phosphoadenylyl-sulfate + lithocholate | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + lithocholate 3-sulfate | - |
? | |
| 3'-phosphoadenylyl-sulfate + taurochenodeoxycholate | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + taurochenodeoxycholate monosulfate + taurochenodeoxycholate disulfate | - |
? | |
| 3'-phosphoadenylyl-sulfate + taurocholate | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + taurocholate monosulfate + taurocholate disulfate + taurocholate trisulfate | - |
? | |
| additional information | 5-cholenic acid-3-beta-ol and 5-beta-cholanic acid-3-beta-ol (isolithocholate) are no substrates | Rattus norvegicus | ? | - |
? | |
| additional information | purified enzyme does not react with estrone or dihydroepiandrosterone | Rattus norvegicus | ? | - |
? | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.026 | - |
isolithocholate | pH 6.5, 37°C | Rattus norvegicus | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Rattus norvegicus | liver enzyme | - |
5.3 |
| Rattus norvegicus | kidney enzyme, isoelectric focusing | - |
5.8 |
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