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Literature summary for 2.8.2.13 extracted from
- Enzymic synthesis of psychosine sulphate (1972), J. Neurochem., 19, 1789-1802.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Mus musculus |  |
| cysteine | - |
Mus musculus | |
| deoxycholate | - |
Mus musculus | |
| EDTA | stimulatory effect | Mus musculus | |
| glutathione | stimulates SO42- incorporation by 23% | Mus musculus | |
| Tween 20 | detergent is necessary for enzyme action in vitro, removing Tween 20 from the incubation medium inhibits enzyme activity 92.5% | Mus musculus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | decreases the incorporation of sulfate ions into psychosine sulfate | Mus musculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
galactosylsphingosine | pH 7.4, 37°C, psychosine | Mus musculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Mus musculus | 5829 | - |
| microsome | - |
Mus musculus | - |
- |
| mitochondrion | - |
Mus musculus | 5739 | - |
| synaptosome | - |
Mus musculus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | divalent ions other than magnesium causes a reduction of enzyme activity | Mus musculus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylylsulfate + galactosylsphingosine | Mus musculus | - |
adenosine 3',5'-bisphosphate + psychosine sulfate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
mouse, normal and Jimpy mutant, maximum enzyme activity at 17-19 days after birth | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Mus musculus | - |
| kidney | - |
Mus musculus | - |
| additional information | hardly any effect in liver | Mus musculus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, microsomes or whole homogenate retains enzyme activity for at least 1 month | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylylsulfate + galactosylsphingosine | - |
Mus musculus | adenosine 3',5'-bisphosphate + psychosine sulfate | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 55 | at 45°C enzyme activity is reduced by 85%, at 55°C activity is lost | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
- |
Mus musculus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.4 | 8 | about 50% of activity maximum at pH 6.4, about 60% of activity maximum at pH 7.4 | Mus musculus |
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Release 2023.1 (January 2023)
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