Activating Compound | Comment | Organism | Structure |
---|---|---|---|
adenosine 3',5'-bisphosphate | - |
Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Rattus norvegicus | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
x * 33000, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + silybin B | Rattus norvegicus | - |
adenosine 3',5'-bisphosphate + silybin B 20-O-sulfate | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
Glycerol | the addition of 10% glycerol (v/v) can double the storage stability of the enzyme | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P17988 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+NTA agarose column chromatography and Superdex 200 gel filtration | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Storage Stability | Organism |
---|---|
-20°C, 50 mM potassium phosphate buffer, 150 mM KCl, 300 mM imidazole, pH7.5, 4 days, 50% loss of activity | Rattus norvegicus |
37°C, 50 mM potassium phosphate buffer, 150 mM KCl, 300 mM imidazole, pH7.5, 6 h, complete loss of activity | Rattus norvegicus |
4°C, 50 mM potassium phosphate buffer, 150 mM KCl, 300 mM imidazole, pH7.5, 1 day, 50% loss of activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + 2-naphthol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + 2-naphthyl sulfate | - |
r | |
3'-phosphoadenylyl sulfate + 4-nitrophenol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate | - |
r | |
3'-phosphoadenylyl sulfate + silybin B | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + silybin B 20-O-sulfate | - |
? | |
additional information | silybin A is completely resistant to the sulfation reaction | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33000, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
aryl sulfotransferase IV | - |
Rattus norvegicus |
ASTIV | - |
Rattus norvegicus |