Cloned (Comment) | Organism |
---|---|
overexpression of SULT1A1 in Escherichia coli strain XL-1 Blue cytosol | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Acetylsalicylate | binds near enough to the substrate site to prevent catalysis but does not affect dissociation of the substrate-enzyme complex | Homo sapiens | |
Ibuprofen | - |
Homo sapiens | |
indomethacin | - |
Homo sapiens | |
meclofenamate | binds near enough to the substrate site to prevent catalysis but does not affect dissociation of the substrate-enzyme complex | Homo sapiens | |
additional information | oxidation of phenolic substrates to carboxylates inhibits the enzyme in a competitive, reversible manner | Homo sapiens | |
naproxen | - |
Homo sapiens | |
nimesulide | binds near enough to the substrate site to prevent catalysis but not affect dissociation of the substrate-enzyme complex | Homo sapiens | |
piroxicam | - |
Homo sapiens | |
salicylate | - |
Homo sapiens | |
sulindac | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic constants of competitive inhibitors | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + 4-nitrophenol | Homo sapiens | - |
adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P50225 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Storage Stability | Organism |
---|---|
-70°C, liver cytosol, 50 mM Tris-HCl, pH 7.8, 0.25 M sucrose, 0.5 mM EDTA, 0.1 mM DTT, and 0.02 mM BHT, no loss of activity during storage | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + 4-nitrophenol | - |
Homo sapiens | adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
phenol sulfotransferase | - |
Homo sapiens |
SULT1A1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000045 | - |
meclofenamate | pH 7.4, 37°C, versus 4-nitrophenol | Homo sapiens | |
0.000056 | - |
meclofenamate | pH 7.4, 37°C, versus 3'-phosphoadenylyl sulfate | Homo sapiens | |
0.00071 | - |
nimesulide | pH 7.4, 37°C, versus 4-nitrophenol | Homo sapiens | |
0.00099 | - |
nimesulide | pH 7.4, 37°C, versus 3'-phosphoadenylyl sulfate | Homo sapiens | |
0.0014 | - |
Acetylsalicylate | pH 7.4, 37°C, versus 4-nitrophenol | Homo sapiens | |
0.0024 | - |
Acetylsalicylate | pH 7.4, 37°C, versus 3'-phosphoadenylyl sulfate | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00011 | - |
pH 7.4, 37°C, versus 4-nitrophenol | Homo sapiens | meclofenamate | |
0.0012 | - |
pH 7.4, 37°C, versus 4-nitrophenol | Homo sapiens | nimesulide | |
0.15 | - |
pH 7.4, 37°C, versus 4-nitrophenol | Homo sapiens | salicylic acid | |
0.38 | - |
pH 7.4, 37°C, versus 4-nitrophenol | Homo sapiens | piroxicam | |
0.39 | - |
pH 7.4, 37°C, versus 4-nitrophenol | Homo sapiens | piroxicam | |
1.3 | - |
pH 7.4, 37°C, versus 4-nitrophenol | Homo sapiens | Acetylsalicylate | |
1.7 | - |
pH 7.4, 37°C, versus 4-nitrophenol | Homo sapiens | naproxen | |
1.9 | - |
pH 7.4, 37°C, versus 4-nitrophenol | Homo sapiens | Ibuprofen | |
3.8 | - |
pH 7.4, 37°C, versus 4-nitrophenol | Homo sapiens | sulindac |