Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.8.2.1 extracted from

  • King, R.S.; Ghosh, A.A.; Wu, J.
    Inhibition of human phenol and estrogen sulfotransferase by certain non-steroidal anti-inflammatory agents (2006), Curr. Drug Metab., 7, 745-753.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.2.1

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of SULT1A1 in Escherichia coli strain XL-1 Blue cytosol Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Acetylsalicylate binds near enough to the substrate site to prevent catalysis but does not affect dissociation of the substrate-enzyme complex Homo sapiens
Ibuprofen
-
 Homo sapiens
indomethacin
-
 Homo sapiens
meclofenamate binds near enough to the substrate site to prevent catalysis but does not affect dissociation of the substrate-enzyme complex Homo sapiens
additional information oxidation of phenolic substrates to carboxylates inhibits the enzyme in a competitive, reversible manner Homo sapiens
naproxen
-
 Homo sapiens
nimesulide binds near enough to the substrate site to prevent catalysis but not affect dissociation of the substrate-enzyme complex Homo sapiens
piroxicam
-
 Homo sapiens
salicylate
-
 Homo sapiens
sulindac
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic constants of competitive inhibitors Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylyl sulfate + 4-nitrophenol Homo sapiens
-
 adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P50225
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Homo sapiens
-

Storage Stability

Storage Stability Organism
-70°C, liver cytosol, 50 mM Tris-HCl, pH 7.8, 0.25 M sucrose, 0.5 mM EDTA, 0.1 mM DTT, and 0.02 mM BHT, no loss of activity during storage Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl sulfate + 4-nitrophenol
-
 Homo sapiens adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
-
 ?

Synonyms

Synonyms Comment Organism
phenol sulfotransferase
-
 Homo sapiens
SULT1A1
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000045
-
 meclofenamate pH 7.4, 37°C, versus 4-nitrophenol Homo sapiens
0.000056
-
 meclofenamate pH 7.4, 37°C, versus 3'-phosphoadenylyl sulfate Homo sapiens
0.00071
-
 nimesulide pH 7.4, 37°C, versus 4-nitrophenol Homo sapiens
0.00099
-
 nimesulide pH 7.4, 37°C, versus 3'-phosphoadenylyl sulfate Homo sapiens
0.0014
-
 Acetylsalicylate pH 7.4, 37°C, versus 4-nitrophenol Homo sapiens
0.0024
-
 Acetylsalicylate pH 7.4, 37°C, versus 3'-phosphoadenylyl sulfate Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00011
-
 pH 7.4, 37°C, versus 4-nitrophenol Homo sapiens meclofenamate
0.0012
-
 pH 7.4, 37°C, versus 4-nitrophenol Homo sapiens nimesulide
0.15
-
 pH 7.4, 37°C, versus 4-nitrophenol Homo sapiens salicylic acid
0.38
-
 pH 7.4, 37°C, versus 4-nitrophenol Homo sapiens piroxicam
0.39
-
 pH 7.4, 37°C, versus 4-nitrophenol Homo sapiens piroxicam
1.3
-
 pH 7.4, 37°C, versus 4-nitrophenol Homo sapiens Acetylsalicylate
1.7
-
 pH 7.4, 37°C, versus 4-nitrophenol Homo sapiens naproxen
1.9
-
 pH 7.4, 37°C, versus 4-nitrophenol Homo sapiens Ibuprofen
3.8
-
 pH 7.4, 37°C, versus 4-nitrophenol Homo sapiens sulindac