Cloned (Comment) | Organism |
---|---|
SULT1,3, cloning from liver, DNA and amino acid sequence determination and analysis, including the promoter region, expression in Escherichia coli strain BL21(DE3) | Squalius cephalus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
genistein | pH 7.0, 20°C, recombinant SULT1,3 | Squalius cephalus | |
0.0044 | - |
daidzein | pH 7.0, 20°C, recombinant SULT1,3 | Squalius cephalus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
x * 35000, recombinant SULT1,3, SDS-PAGE | Squalius cephalus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Squalius cephalus | A0FIP8 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | low SULT1,3 expression level | Squalius cephalus | - |
gill | low SULT1,3 expression level | Squalius cephalus | - |
gonad | low SULT1,3 expression level | Squalius cephalus | - |
kidney | high SULT1,3 expression level | Squalius cephalus | - |
liver | high SULT1,3 expression level, mature male fish display higher hepatic SULT1,3 mRNA expression compared to female fish | Squalius cephalus | - |
additional information | quantitative tissue expression analysis of SULT1,3, overview | Squalius cephalus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + 4-nitrophenol | - |
Squalius cephalus | adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate | - |
? | |
3'-phosphoadenylyl sulfate + daidzein | - |
Squalius cephalus | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylyl sulfate + dopamine | low activity | Squalius cephalus | adenosine 3',5'-bisphosphate + dopamine sulfate | - |
? | |
3'-phosphoadenylyl sulfate + genistein | - |
Squalius cephalus | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylyl sulfate + nonyl phenol | low activity | Squalius cephalus | adenosine 3',5'-bisphosphate + nonyl phenyl sulfate | - |
? | |
3'-phosphoadenylyl sulfate + octyl phenol | low activity | Squalius cephalus | adenosine 3',5'-bisphosphate + octyl phenyl sulfate | - |
? | |
additional information | no activity with the synthetic estrogens ethynyl estradiol and diethylstilbestrol or endogenous substrates estrone, 17beta-estradiol, dehydroepiandrosterone, and the thyroid hormones 3,3',5-triiodo-L-thyronine T3 and thyronine T4, the enzyme contains two conserved co-substrate binding motifs for 30-phosphoadenosine 50-phosphosulfate: RKGxxGDWKxxFT and YPKSGTxW | Squalius cephalus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35000, recombinant SULT1,3, SDS-PAGE | Squalius cephalus |
More | the enzyme contains two conserved co-substrate binding motifs for 30-phosphoadenosine 50-phosphosulfate: RKGxxGDWKxxFT and YPKSGTxW | Squalius cephalus |
Synonyms | Comment | Organism |
---|---|---|
sulfotransferase 1,3 | - |
Squalius cephalus |
SULT1,3 | - |
Squalius cephalus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Squalius cephalus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Squalius cephalus |