Cloned (Comment) | Organism |
---|---|
expression of pTrc99A-bSULT1A1 in Escherichia coli DH5alpha | Bos taurus |
SULT1A1, expression in Escherichia coli strain DH5alpha | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acyl-CoA | weak inhibition | Bos taurus | |
CoA | reversible covalent inhibition, the inactivation of the dimeric enzyme is stoichiometric, binding structure and mechanism, overview | Bos taurus | |
coenzyme A | - |
Bos taurus | |
palmitoyl-CoA | - |
Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + a phenol | Bos taurus | - |
adenosine 3',5'-bisphosphate + an aryl sulfate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | P50227 | - |
- |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose column chromatography | Bos taurus |
recombinant SULT1A1 from Escherichia coli strain DH5alpha by anion exchange chromatography and gel filtration | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + 7-hydroxycoumarin | - |
Bos taurus | adenosine 3',5'-bisphosphate + 7-hydroxycoumarin sulfate | - |
? | |
3'-phosphoadenylyl sulfate + 7-hydroxycoumarin | SULT molecular dynamic, substrate 3'-phosphoadenylyl sulfate, i.e. PAPS, induces a structural change, overview | Bos taurus | adenosine 3',5'-bisphosphate + 7-hydroxycoumarin sulfate | - |
? | |
3'-phosphoadenylyl sulfate + a phenol | - |
Bos taurus | adenosine 3',5'-bisphosphate + an aryl sulfate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | molecular modeling of the enzyme structure using the crystal structure PDB ID 1LS6 | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
phenol sulfotransferase | - |
Bos taurus |
SULT1A1 | - |
Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Bos taurus | |
0.0021 | 0.0028 | CoA | pH 7.4, 25°C, recombinant SULT1A1, method B | Bos taurus | |
0.00573 | - |
CoA | pH 7.4, 25°C, recombinant SULT1A1, method A | Bos taurus | |
0.00577 | - |
coenzyme A | - |
Bos taurus |