Literature summary for 2.8.2.1 extracted from

Chodavarapu, S.; Hertema, H.; Huynh, T.; Odette, J.; Miller, R.; Fullerton, A.; Alkirwi, J.; Hartsfield, D.; Padmanabhan, K.; Woods, C.; Beckmann, J.D.
Reversible covalent inhibition of a phenol sulfotransferase by coenzyme A (2007), Arch. Biochem. Biophys., 457, 197-204.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression of pTrc99A-bSULT1A1 in Escherichia coli DH5alpha	Bos taurus
SULT1A1, expression in Escherichia coli strain DH5alpha	Bos taurus

Inhibitors

Inhibitors	Comment	Organism	Structure
acyl-CoA	weak inhibition	Bos taurus
CoA	reversible covalent inhibition, the inactivation of the dimeric enzyme is stoichiometric, binding structure and mechanism, overview	Bos taurus
coenzyme A	-	Bos taurus
palmitoyl-CoA	-	Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3'-phosphoadenylyl sulfate + a phenol	Bos taurus	-	adenosine 3',5'-bisphosphate + an aryl sulfate	-	?

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	P50227	-	-

Purification (Commentary)

Purification (Comment)	Organism
DEAE-cellulose column chromatography	Bos taurus
recombinant SULT1A1 from Escherichia coli strain DH5alpha by anion exchange chromatography and gel filtration	Bos taurus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3'-phosphoadenylyl sulfate + 7-hydroxycoumarin	-	Bos taurus	adenosine 3',5'-bisphosphate + 7-hydroxycoumarin sulfate	-	?
3'-phosphoadenylyl sulfate + 7-hydroxycoumarin	SULT molecular dynamic, substrate 3'-phosphoadenylyl sulfate, i.e. PAPS, induces a structural change, overview	Bos taurus	adenosine 3',5'-bisphosphate + 7-hydroxycoumarin sulfate	-	?
3'-phosphoadenylyl sulfate + a phenol	-	Bos taurus	adenosine 3',5'-bisphosphate + an aryl sulfate	-	?

Subunits

Subunits	Comment	Organism
More	molecular modeling of the enzyme structure using the crystal structure PDB ID 1LS6	Bos taurus

Synonyms

Synonyms	Comment	Organism
phenol sulfotransferase	-	Bos taurus
SULT1A1	-	Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Bos taurus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Bos taurus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
additional information	-	additional information	inhibition kinetics	Bos taurus
0.0021	0.0028	CoA	pH 7.4, 25°C, recombinant SULT1A1, method B	Bos taurus
0.00573	-	CoA	pH 7.4, 25°C, recombinant SULT1A1, method A	Bos taurus
0.00577	-	coenzyme A	-	Bos taurus

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Release 2023.1 (January 2023)