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Literature summary for 2.8.2.1 extracted from

  • Pai, T.G.; Ohkimoto, K.; Sakakibara, Y.; Suiko, M.; Sugahara, T.; Liu, M.C.
    Manganese stimulation and stereospecificity of the Dopa (3,4-dihydroxyphenylalanine)/tyrosine-sulfating activity of human monoamine-form phenol sulfotransferase. Kinetic studies of the mechanism using wild-type and mutant enzymes (2002), J. Biol. Chem., 277, 43813-43820.
    View publication on PubMed
Search for more literature for 2.8.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type M-PST and mutant D86A in Escherichia coli BL21 Homo sapiens

Protein Variants

Protein Variants Comment Organism
D86A M-PST mutant by site-directed mutagenesis, reduced activity Homo sapiens
D86A no stimulating effect by Mn2+, probably due to abolished substrate/Mn2+-complex binding via Asp86 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ stimulates, higher effect with D-isomers of 3,4-dihydroxyphenylalanine and tyrosine than with the L-isomer as substrates, highly stereospecific, kinetics Homo sapiens
Mn2+ complex formation with the substrate enhances the stimulating effect Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 2 forms: simple phenol sulfating P-PST, monoamine sulfating M-PST
-

Reaction

Reaction Comment Organism Reaction ID
3'-phosphoadenylyl sulfate + a phenol = adenosine 3',5'-bisphosphate + an aryl sulfate Asp86 is located at the active site in the intercalating mobile loop and responsible for substrate binding Homo sapiens
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.059
-
 purified M-PST mutantD86A, substrate dopamine Homo sapiens
0.063
-
 purified M-PST mutantD86A, substrate dopamine + Mn2+ Homo sapiens
0.5
-
 purified M-PST wild-type enzyme, substrate dopamine Homo sapiens
1.13
-
 purified M-PST wild-type enzyme, substrate dopamine + Mn2+ Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl sulfate + a phenol
-
 Homo sapiens adenosine 3',5'-bisphosphate + an aryl sulfate
-
 ?
3'-phosphoadenylylsulfate + D-3,4-dihydroxyphenylalanine M-PST Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + D-3,4-dihydroxyphenylalanine i.e. D-Dopa Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + D-3,4-dihydroxyphenylalanine preferred isomer, stereospecific Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + dopamine M-PST Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + L-3,4-dihydroxyphenylalanine low activity Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + L-3,4-dihydroxyphenylalanine i.e. L-dopa Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + L-3,4-dihydroxyphenylalanine M-PST Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?