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Literature summary for 2.8.2.1 extracted from

  • Sekura, R.D.; Duffel, M.W.; Jakoby, W.B.
    Aryl sulfotransferases (1981), Methods Enzymol., 77, 197-206.
    View publication on PubMed
Search for more literature for 2.8.2.1

General Stability

General Stability Organism
transferase II is unstable to isoelectric focusing Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
2,6-Dichloro-4-nitrophenol
-
 Rattus norvegicus
Pentachlorophenol
-
 Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
KCl 50 mM, 60% inhibition of isoenzyme A, slight activation of isoenzyme B, aryl sulfotransferase I and II are activated appreciably in the presence of 0.5 M KCl or NaCl Rattus norvegicus
additional information
-
 Rattus norvegicus
NaCl aryl sulfotransferase I and II are activated appreciably in the presence of 0.5 M NaCl or KCl Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33500
-
 2 * 33500, arylsulfotransferase III, SDS-PAGE Rattus norvegicus
61000
-
 arylsulfotransferase III, gel filtration Rattus norvegicus
64000
-
 arylsulfotransferase I or II, gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
arylsulfotransferase I, II, III Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.25
-
 arylsulfotransferase I Rattus norvegicus
0.282
-
 arylsulfotransferase II Rattus norvegicus

Storage Stability

Storage Stability Organism
-80°C, transferase III may deteriorate at any stage of purification Rattus norvegicus
4°C, sodium phosphate, pH 7.0, 0.25 M sucrose, 5 mM 2-mercaptoethanol, 3 mM NaN3, protein concentration 0.5-1 mg/ml, transferase I, II lose about 5% of their activity per week Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl sulfate + a phenol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + an aryl sulfate
-
 ?
3'-phosphoadenylylsulfate + 2-naphthol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 2-naphthyl sulfate
-
 ?
3'-phosphoadenylylsulfate + 4-chlorophenol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 4-chlorophenyl sulfate
-
 ?
3'-phosphoadenylylsulfate + 4-methoxyphenol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 4-methoxyphenyl sulfate
-
 ?
3'-phosphoadenylylsulfate + phenol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + phenyl sulfate
-
 ?
additional information activity with various substrates at different pH values Rattus norvegicus ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 64000, arylsulfotransferase I or II Rattus norvegicus
dimer 2 * 33500, arylsulfotransferase III, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Rattus norvegicus
0.113
-
 phenol isoenzyme I Rattus norvegicus
0.122
-
 phenol isoenzyme II Rattus norvegicus
0.667
-
 4-Chlorophenol isoenzyme II Rattus norvegicus
0.783
-
 2-naphthol isoenzyme I Rattus norvegicus
0.8
-
 4-Chlorophenol isoenzyme I Rattus norvegicus
0.9
-
 2-naphthol isoenzyme II Rattus norvegicus
1.55
-
 4-methoxyphenol isoenzyme I Rattus norvegicus
2
-
 4-methoxyphenol isoenzyme II Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 with substrate 2-naphthol, aryl sulfotransferase I and II Rattus norvegicus
6.5
-
 with substrate 2-naphthol, aryl sulfotransferase II Rattus norvegicus