Literature summary for 2.8.1.7 extracted from

Pandey, A.; Gordon, D.M.; Pain, J.; Stemmler, T.L.; Dancis, A.; Pain, D.
Frataxin directly stimulates mitochondrial cysteine desulfurase by exposing substrate-binding sites, and a mutant Fe-S cluster scaffold protein with frataxin-bypassing ability acts similarly (2013), J. Biol. Chem., 288, 36773-36786.

Search for more literature for 2.8.1.7

Activating Compound

Activating Compound	Comment	Organism	Structure
frataxin	frataxin or a mutant Fe-S scaffold protein (Isu1Sup) with frataxin-bypassing ability stimulates L-cysteine binding to the enzyme	Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 (DE3) Codon Plus cells	Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
K263A	inactive	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Saccharomyces cerevisiae	5739	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
55000	-	x * 55000, SDS-PAGE	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-cysteine + acceptor	Saccharomyces cerevisiae	-	L-alanine + S-sulfanyl-acceptor	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA agarosecolumn chromatography	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-cysteine + acceptor	-	Saccharomyces cerevisiae	L-alanine + S-sulfanyl-acceptor	-	?

Subunits

Subunits	Comment	Organism
?	x * 55000, SDS-PAGE	Saccharomyces cerevisiae

Synonyms

Synonyms	Comment	Organism
Nfs1	-	Saccharomyces cerevisiae

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)