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Literature summary for 2.8.1.7 extracted from

  • Turowski, V.R.; Busi, M.V.; Gomez-Casati, D.F.
    Structural and functional studies of the mitochondrial cysteine desulfurase from Arabidopsis thaliana (2012), Mol. Plant, 5, 1001-1010.
    View publication on PubMed
Search for more literature for 2.8.1.7

Activating Compound

Activating Compound Comment Organism Structure
frataxin frataxin modulates isoform Nfs1m kinetics, increasing Vmax and decreasing the S0.5 value for L-cysteine Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) RIL cells Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast isoform Nfs2 Arabidopsis thaliana 9507
-
mitochondrion isoform Nfs1 Arabidopsis thaliana 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 x * 47000, isoform Nfs1, SDS-PAGE Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O49543 isoform Nfs1; ecotype Columbia Col-0
-
Arabidopsis thaliana Q93WX6 isoform Nfs2; ecotype Columbia Col-0
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap chelating column chromatography, and gel filtration Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cysteine + [enzyme]-cysteine
-
 Arabidopsis thaliana L-alanine + [enzyme]-S-sulfanylcysteine
-
 ?

Subunits

Subunits Comment Organism
? x * 47000, isoform Nfs1, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
Nfs1 isoform Arabidopsis thaliana
Nfs2 isoform Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Arabidopsis thaliana