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Literature summary for 2.8.1.7 extracted from

  • Loiseau, L.; Ollagnier-de-Choudens, S.; Nachin, L.; Fontecave, M.; Barras, F.
    Biogenesis of Fe-S cluster by the bacterial Suf system: SufS and SufE form a new type of cysteine desulfurase (2003), J. Biol. Chem., 278, 38352-38359.
    View publication on PubMed
Search for more literature for 2.8.1.7

Activating Compound

Activating Compound Comment Organism Structure
SufE binding of SufE, a member of the SUF protein system, to SufS stimulates the cysteine desulfurase activity of SufS by 50fold Escherichia coli
SufE binding of SufE, a member of the SUF protein system, to SufS stimulates the cysteine desulfurase activity of SufS by 50fold Dickeya chrysanthemi

Cloned(Commentary)

Cloned (Comment) Organism
-
 Dickeya chrysanthemi

Protein Variants

Protein Variants Comment Organism
C369S no enzyme activity Dickeya chrysanthemi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2
-
 L-selenocysteine pH 8, 37°C Dickeya chrysanthemi

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Escherichia coli 5737
-
cytoplasm
-
 Dickeya chrysanthemi 5737
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-cysteine + [enzyme]-cysteine Escherichia coli
-
 L-alanine + [enzyme]-S-sulfanylcysteine
-
 ?
L-cysteine + [enzyme]-cysteine Dickeya chrysanthemi
-
 L-alanine + [enzyme]-S-sulfanylcysteine
-
 ?
L-cysteine + [enzyme]-cysteine Escherichia coli TG1
-
 L-alanine + [enzyme]-S-sulfanylcysteine
-
 ?

Organism

Organism UniProt Comment Textmining
Dickeya chrysanthemi
-
-
-
Escherichia coli
-
 TG1
-
Escherichia coli TG1
-
 TG1
-

Purification (Commentary)

Purification (Comment) Organism
-
 Dickeya chrysanthemi

Reaction

Reaction Comment Organism Reaction ID
L-cysteine + acceptor = L-alanine + S-sulfanyl-acceptor mechanism Escherichia coli
a
L-cysteine + acceptor = L-alanine + S-sulfanyl-acceptor mechanism Dickeya chrysanthemi
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cysteine + [enzyme]-cysteine
-
 Escherichia coli L-alanine + [enzyme]-S-sulfanylcysteine
-
 ?
L-cysteine + [enzyme]-cysteine
-
 Dickeya chrysanthemi L-alanine + [enzyme]-S-sulfanylcysteine
-
 ?
L-cysteine + [enzyme]-cysteine
-
 Escherichia coli TG1 L-alanine + [enzyme]-S-sulfanylcysteine
-
 ?
L-selenocysteine
-
 Escherichia coli L-alanine + selenium
-
 ?
L-selenocysteine
-
 Dickeya chrysanthemi L-alanine + selenium
-
 ?
L-selenocysteine
-
 Escherichia coli TG1 L-alanine + selenium
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Dickeya chrysanthemi