Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli B834(DE3) cells | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dethiobiotin + [S] + 2 S-adenosyl-L-methionine | - |
Escherichia coli | biotin + 2 L-methionine + 2 5'-deoxyadenosine | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
BioB | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | overexpression of biotin synthase is required for efficient generation of sulfur-35 labeled biotin in Escherichia coli. Coexpression of both BioB and the biotin ligase, BirA, is required for efficient biotinylation of biotin carboxy carrier protein-79 | Escherichia coli |