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Literature summary for 2.8.1.4 extracted from
- Mammalian tRNA sulfurtransferase: properties of the enzyme in rat liver (1978), Nucleic Acids Res., 5, 599-613.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cysteine | inhibits reaction with 3-mercaptopyruvate | Rattus norvegicus |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-cysteine + 'activated' tRNA | Rattus norvegicus | - |
L-serine + tRNA containing a thionucleotide | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
Wistar albino | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adrenal gland | - |
Rattus norvegicus | - |
| brain | cerebral cortex, lowest activity | Rattus norvegicus | - |
| heart | - |
Rattus norvegicus | - |
| kidney | - |
Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
| lung | - |
Rattus norvegicus | - |
| muscle | highest activity | Rattus norvegicus | - |
| testis | - |
Rattus norvegicus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -60°C, instable on storage, overnight storage results in 50% lost of activity | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-mercaptopyruvate + activated tRNA | reaction product is sensitive to deacylation | Rattus norvegicus | ? + S-tRNA | - |
? | |
| L-cysteine + 'activated' tRNA | - |
Rattus norvegicus | L-serine + tRNA containing a thionucleotide | - |
r | |
| L-cysteine + Bos taurus 'activated' tRNA | calf liver tRNA better substrate than rat liver tRNA | Rattus norvegicus | L-serine + tRNA containing a thionucleotide | - |
r | |
| L-cysteine + E. coli 'activated' tRNA | sulfur deficient tRNA | Rattus norvegicus | L-serine + tRNA containing a thionucleotide | - |
r | |
| L-cysteine + Rattus norvegicus brain 'activated' tRNA | better substrate than liver tRNA | Rattus norvegicus | L-serine + tRNA containing a thionucleotide | - |
r | |
| L-cysteine + Rattus norvegicus liver 'activated' tRNA | poorest substrate | Rattus norvegicus | L-serine + tRNA containing a thionucleotide | - |
r | |
| additional information | only tRNAs are effective sulfur acceptors, little or no reaction with rat liver rRNA, salmon sperm DNA, polyuridylic acid and polyadenylic acid | Rattus norvegicus | ? | - |
? | |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 37 | at 10°C rate of sulfur transfer being 1/3 that at 37°C | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | - |
Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | about 75% of activity maximum at pH 7.0, about 55% of activity maximum at pH 9.0 | Rattus norvegicus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
inactivated | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Rattus norvegicus | |
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Release 2023.1 (January 2023)
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