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Literature summary for 2.8.1.4 extracted from

  • Harris, C.L.; Titchener, E.B.
    Sulfur-deficient transfer ribonucleic acid. The natural substrate for ribonucleic acid sulfurtransferase from Escherichia coli (1971), Biochemistry, 10, 4207-4212.
    View publication on PubMed
Search for more literature for 2.8.1.4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0027
-
 tRNA pH 7.5, 37°C, sulfur deficient tRNA Escherichia coli
0.26
-
 L-cysteine pH 7.5, 37°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-cysteine + 'activated' tRNA Escherichia coli sulfur deficient tRNA is the natural substrate L-serine + tRNA containing a thionucleotide
-
 r
L-cysteine + 'activated' tRNA Escherichia coli HfrC sulfur deficient tRNA is the natural substrate L-serine + tRNA containing a thionucleotide
-
 r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 HfrC
-
Escherichia coli HfrC
-
 HfrC
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cysteine + 'activated' tRNA
-
 Escherichia coli L-serine + tRNA containing a thionucleotide
-
 r
L-cysteine + 'activated' tRNA sulfur deficient tRNA is the natural substrate Escherichia coli L-serine + tRNA containing a thionucleotide
-
 r
L-cysteine + 'activated' tRNA
-
 Escherichia coli HfrC L-serine + tRNA containing a thionucleotide
-
 r
L-cysteine + 'activated' tRNA sulfur deficient tRNA is the natural substrate Escherichia coli HfrC L-serine + tRNA containing a thionucleotide
-
 r
L-cysteine + E. coli 'activated' tRNA sulfur deficient tRNA is the natural substrate Escherichia coli L-serine + tRNA containing a thionucleotide
-
 ?
L-cysteine + E. coli 'activated' tRNA sulfur deficient tRNA is the natural substrate Escherichia coli HfrC L-serine + tRNA containing a thionucleotide
-
 ?
L-cysteine + tRNA
-
 Escherichia coli ?
-
 ?
L-cysteine + tRNA
-
 Escherichia coli HfrC ?
-
 ?
additional information 2-thiocytidine is the major thionucleotide formed in vitro, 4-thiouridine is the major product in vivo Escherichia coli ?
-
 ?
additional information 2-thiocytidine is the major thionucleotide formed in vitro, 4-thiouridine is the major product in vivo Escherichia coli HfrC ?
-
 ?

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli