Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.8.1.4 extracted from

  • Abrell, J.W.; Kaufman, E.E.; Lipsett, M.N.
    The biosynthesis of 4-thiouridylate. Separation and purification of two enzymes in the transfer ribonucleic acid-sulfurtransferase system (1971), J. Biol. Chem., 246, 294-301.
    View publication on PubMed
Search for more literature for 2.8.1.4

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ inhibits the synthesis of s4U Escherichia coli
Co2+ inhibits the synthesis of s4U Escherichia coli
Fe2+ inhibits the synthesis of s4U Escherichia coli
Ni2+ inhibits the synthesis of s4U Escherichia coli
Zn2+ inhibits the synthesis of s4U Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012 0.0016 L-cysteine pH 7.8, 37°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-cysteine + 'activated' tRNA Escherichia coli enzyme is part of the sulfur-transferase system which forms 4-thiouridylate in tRNA L-serine + tRNA containing a thionucleotide
-
 r
L-cysteine + 'activated' tRNA Escherichia coli B / ATCC 11303 enzyme is part of the sulfur-transferase system which forms 4-thiouridylate in tRNA L-serine + tRNA containing a thionucleotide
-
 r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 B
-
Escherichia coli B / ATCC 11303
-
 B
-

Purification (Commentary)

Purification (Comment) Organism
sulfurtransferase system with 2 enzymes Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, partly purified pooled protein fractions can be stored indefinitely for periods up to several months Escherichia coli
4°C, addition of toluene, can be stored indefinitely for periods up to several months Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cysteine + 'activated' tRNA
-
 Escherichia coli L-serine + tRNA containing a thionucleotide
-
 r
L-cysteine + 'activated' tRNA enzyme is part of the sulfur-transferase system which forms 4-thiouridylate in tRNA Escherichia coli L-serine + tRNA containing a thionucleotide
-
 r
L-cysteine + 'activated' tRNA
-
 Escherichia coli B / ATCC 11303 L-serine + tRNA containing a thionucleotide
-
 r
L-cysteine + 'activated' tRNA enzyme is part of the sulfur-transferase system which forms 4-thiouridylate in tRNA Escherichia coli B / ATCC 11303 L-serine + tRNA containing a thionucleotide
-
 r
L-cysteine + E. coli 'activated' tRNA Escherichia coli B tRNA and irradiated Escherichia coli tRNA, S-poor Escherichia coli tRNA also acts as RNA acceptors Escherichia coli L-serine + tRNA containing a thionucleotide
-
 r
L-cysteine + E. coli 'activated' tRNA Escherichia coli B tRNA and irradiated Escherichia coli tRNA, S-poor Escherichia coli tRNA also acts as RNA acceptors Escherichia coli B / ATCC 11303 L-serine + tRNA containing a thionucleotide
-
 r
L-cysteine + poly A-2poly U
-
 Escherichia coli L-serine + S-tRNA
-
 ?
L-cysteine + poly A-2poly U
-
 Escherichia coli B / ATCC 11303 L-serine + S-tRNA
-
 ?
L-cysteine + poly A-poly U
-
 Escherichia coli L-serine + S-tRNA
-
 ?
L-cysteine + poly A-poly U
-
 Escherichia coli B / ATCC 11303 L-serine + S-tRNA
-
 ?
L-cysteine + poly G,U G:U = 2 Escherichia coli L-serine + S-tRNA
-
 ?
L-cysteine + Saccharomyces cerevisiae 'activated' tRNA same activity with baker's yeast tRNA as RNA acceptor Escherichia coli L-serine + tRNA containing a thionucleotide
-
 r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.4
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli
additional information beta-mercaptopyruvate is inactive either as a cofactor or as a sulfur donor Escherichia coli