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Literature summary for 2.8.1.3 extracted from
- Purification and steady-state kinetic analysis of yeast thiosulfate reductase (1979), Arch. Biochem. Biophys., 195, 211-222.
General Stability
| General Stability | Organism |
|---|---|
| freezing and thawing during the later stages of purification destabilize | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| benzene thiosulfonate | competitive with both substrates | Saccharomyces cerevisiae |  |
| oxidized glutathione | product inhibition, competitive with glutathione | Saccharomyces cerevisiae | |
| sulfate | - |
Saccharomyces cerevisiae | |
| Sulfide | product inhibition, competitive versus both substrates | Saccharomyces cerevisiae | |
| sulfite | product inhibition, competitive versus both substrates | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17000 | - |
SDS-PAGE, gel filtration | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thiosulfate + glutathione | Saccharomyces cerevisiae | - |
sulfite + glutathione disulfide + sulfide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
Red Star brand | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 70fold | Saccharomyces cerevisiae |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| thiosulfate + 2 glutathione = sulfite + glutathione disulfide + sulfide | mechanism, substrates add in a random fashion | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 62 | - |
- |
Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -35°C, concentrated enzyme in solution of 50 mM Tris, pH 8.0, 0.5 mM NaS2O3, 50% glycerol, stable for at least 4 months | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-cysteine + glutathione | - |
Saccharomyces cerevisiae | ? | - |
? | |
| additional information | the enzyme has two distinct closely situated substrate binding sites, one for compounds with an RSO3-structure and one for the sulfhydryl substrate | Saccharomyces cerevisiae | ? | - |
? | |
| thiosulfate + glutathione | - |
Saccharomyces cerevisiae | sulfite + glutathione disulfide + sulfide | - |
? | |
| thiosulfate + glutathione | - |
Saccharomyces cerevisiae | sulfite + glutathione disulfide + sulfide | the primary product is glutathione hydrodisulfide, which reacts with glutathione to give oxidized glutathione and sulfide | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 17000, SDS-PAGE | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Saccharomyces cerevisiae |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Saccharomyces cerevisiae | isoelectric focusing using ampholytes pH 4-6 and pH 2-10 | - |
5.1 |
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