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Literature summary for 2.8.1.15 extracted from

  • Chen, M.; Narai, S.; Omura, N.; Shigi, N.; Chimnaronk, S.; Tanaka, Y.; Yao, M.
    Identification Crystallographic study of the 2-thioribothymidine-synthetic complex TtuA-TtuB from Thermus thermophilus (2016), Acta Crystallogr. Sect. F, 72, 777.781.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.1.15

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Thermus thermophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
vapour-diffusion method at 293 K under anaerobic conditions, 2-thioribothymidinesynthetic complex TtuA–TtuB Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyluridine54 in tRNA + acceptor Thermus thermophilus
-
 AMP + diphosphate + 5-methyl-2-thiouridine54 in tRNA + [TtuB sulfur-carrier protein] + reduced acceptor
-
 ?
ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyluridine54 in tRNA + acceptor Thermus thermophilus DSM 7039
-
 AMP + diphosphate + 5-methyl-2-thiouridine54 in tRNA + [TtuB sulfur-carrier protein] + reduced acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q72LF3
-
-
Thermus thermophilus DSM 7039 Q72LF3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyluridine54 in tRNA + acceptor
-
 Thermus thermophilus AMP + diphosphate + 5-methyl-2-thiouridine54 in tRNA + [TtuB sulfur-carrier protein] + reduced acceptor
-
 ?
ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyluridine54 in tRNA + acceptor
-
 Thermus thermophilus DSM 7039 AMP + diphosphate + 5-methyl-2-thiouridine54 in tRNA + [TtuB sulfur-carrier protein] + reduced acceptor
-
 ?

Synonyms

Synonyms Comment Organism
TTC0106
-
 Thermus thermophilus
TtuA
-
 Thermus thermophilus