Protein Variants | Comment | Organism |
---|---|---|
C104A | mutation completely hinders the ability of MnmA along with YrvO to synthesize s2U in vivo | Bacillus subtilis |
C200A | mutation completely hinders the ability of MnmA along with YrvO to synthesize s2U in vivo | Bacillus subtilis |
C51A | mutation completely hinders the ability of MnmA along with YrvO to synthesize s2U in vivo | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | O35020 | - |
- |
Bacillus subtilis 168 | O35020 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a [protein]-S-sulfanyl-L-cysteine + uridine34 in tRNA + ATP + reduced acceptor | - |
Bacillus subtilis | a [protein]-L-cysteine + 2-thiouridine34 in tRNA + AMP + diphosphate + acceptor | enzyme adenylates and subsequently thiolates tRNA to form the s2U modification | ? | |
a [protein]-S-sulfanyl-L-cysteine + uridine34 in tRNA + ATP + reduced acceptor | - |
Bacillus subtilis 168 | a [protein]-L-cysteine + 2-thiouridine34 in tRNA + AMP + diphosphate + acceptor | enzyme adenylates and subsequently thiolates tRNA to form the s2U modification | ? |
Synonyms | Comment | Organism |
---|---|---|
MnmA | - |
Bacillus subtilis |
General Information | Comment | Organism |
---|---|---|
physiological function | in a conditional Bacillus subtilis knockout strain s2U abundance correlates with tRNA-uridine 2-sulfurtransferase MnmA expression, and MnmA is able to complement Escherichia coli MnmA-deficient strains. Cysteine desulfurase IscS is able to transfer sulfur directly to MnmA in presence of ATP. Both proteins are sufficient for s2U biosynthesis in a pathway independent of the one used in Escherichia coli | Bacillus subtilis |