Cloned (Comment) | Organism |
---|---|
MOCS2A and MOCS2B genotyping, recombinant expression of human subunits MOCS2A and MOCS2B in Escherichia coli strain BL21(DE3), functional complementation of Escherichia coli moaD and moaE mutants. In vitro translation and mutagenesis experiments of MOCS2A and MOCS2B | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E168K | naturally occuring substitution in MOCS2B, the E168K mutation, identified in a severely affected patient, attenuates binding of precursor Z, the MPT synthase tetramer is readily formed in mixtures of MOCS2B-E168K with equimolar amounts of MOCS2A | Homo sapiens |
additional information | a deletion of Ala150 is introduced into MOCS2B, attempts to purify MOCS2B-A150DELTA fails because the protein forms inclusion bodies upon expression in the Escherichia coli cells. Construction of a MOCS2B variant lacking the first 43 amino acid residues, the DELTA1-43 variant of MOCS2B is unstable because the majority of the protein was rapidly degraded during or after purification | Homo sapiens |
V7F | naturally occuring substitution in MOCS2A, identified in a patient with an unusual mild form of the disease, the mutation weakens the interaction between MOCS2A and MOCS2B, the MOCS2A-V7F variant does not form a complex with MOCS2B in either its carboxylated or thiocarboxylated form, and the mutant MPT synthase shows 90% reduced activity compared to the wild-type enzyme | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
9800 | - |
2 * 9800, subunit MOCS2A, + 2 * 20800, subunit MCS2B, SDS-PAGE | Homo sapiens |
20800 | - |
2 * 9800, subunit MOCS2A, + 2 * 20800, subunit MCS2B, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclic pyranopterin phosphate + 2 [molybdopterin-synthase sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + H2O | Homo sapiens | - |
molybdopterin + 2 molybdopterin-synthase sulfur-carrier protein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O96007 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant human subunits MOCS2A and MOCS2B from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation and gel filtration. The separately purified subunits readily assemble into a functional MPT synthase tetramer | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclic pyranopterin phosphate + 2 [molybdopterin-synthase sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + H2O | - |
Homo sapiens | molybdopterin + 2 molybdopterin-synthase sulfur-carrier protein | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | 2 * 9800, subunit MOCS2A, + 2 * 20800, subunit MCS2B, SDS-PAGE | Homo sapiens |
More | human MPT synthase contains the subunits MOCS2A and MOCS2B | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
MPT synthase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | characterization of mutants identified in group B patients of molybdenum cofactor deficiency, molecular mechanism leading to human molybdenum cofactor deficiency, overview | Homo sapiens |
additional information | the rate of conversion of precursor Z to MPT by the human enzyme is slower than that of the eubacterial homologue from Escherichia coli | Homo sapiens |