Protein Variants | Comment | Organism |
---|---|---|
additional information | overexpression of His-tagged proteins ThiF, NifS, ThiO, and ThiSG Escherichia coli BL21and incubation with L-cysteine in the presence of dithiothreitol and ATP to form ThiS-COSH. This is then added to 1-deoxy-D-xylulose 5-phosphate and glycine in the presence of ThiO and ThiG to produce the product of the thiazole synthase-catalyzed reaction. Reaction product is a dearomatized thiazole tautomer | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | O31618 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS] | - |
Bacillus subtilis | 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O | product is a dearomatized thiazole tautomer, not thiazole phosphate | ? |