Literature summary for 2.8.1.1 extracted from

Giuliani, M.C.; Jourlin-Castelli, C.; Leroy, G.; Hachani, A.; Giudici-Orticoni, M.T.
Characterization of a new periplasmic single-domain rhodanese encoded by a sulfur-regulated gene in a hyperthermophilic bacterium Aquifex aeolicus (2010), Biochimie, 92, 388-397.

Search for more literature for 2.8.1.1

Inhibitors

Inhibitors	Comment	Organism	Structure
iodoacetamide	complete inhibition at 20 mM	Aquifex aeolicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1	-	thiosulfate	apparent value, in 100 mM Tris-HCl, pH 9.0, 10 mM KCN, at 85°C	Aquifex aeolicus
1.7	-	cyanide	apparent value, in 100 mM Tris-HCl, pH 9.0, 10 mM KCN, at 85°C	Aquifex aeolicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
periplasm	-	Aquifex aeolicus	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
15000	-	4 * 15000, calculated from amino acid sequence	Aquifex aeolicus
18000	-	4 * 18000, SDS-PAGE	Aquifex aeolicus
65000	-	SDS-PAGE	Aquifex aeolicus

Organism

Organism	UniProt	Comment	Textmining
Aquifex aeolicus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	no activity with mercaptopyruvate as sulfur donor	Aquifex aeolicus	?	-	?
thiosulfate + cyanide	-	Aquifex aeolicus	sulfite + thiocyanate	-	?

Subunits

Subunits	Comment	Organism
homotetramer	4 * 18000, SDS-PAGE	Aquifex aeolicus
homotetramer	4 * 15000, calculated from amino acid sequence	Aquifex aeolicus

Synonyms

Synonyms	Comment	Organism
Aq-1599	-	Aquifex aeolicus
RhdB2	-	Aquifex aeolicus
rhodanese	-	Aquifex aeolicus
thiosulfate cyanide sulfurtransferase	-	Aquifex aeolicus
thiosulfate:cyanide transferase	-	Aquifex aeolicus
TST	-	Aquifex aeolicus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
400	-	thiosulfate	in 100 mM Tris-HCl, pH 9.0, 10 mM KCN, at 85°C	Aquifex aeolicus

Expression

Organism	Comment	Expression
Aquifex aeolicus	the expression of the aq-1599 gene is induced in the presence of elemental sulfur	up

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Release 2023.1 (January 2023)