Cloned (Comment) | Organism |
---|---|
expression in a cell free Escherichia coli system by coupled transcription/translation | Bos taurus |
Protein Variants | Comment | Organism |
---|---|---|
Arg | one additional Arg residue at the 3'-end followed by a TAA stop codon, no significant changes to the wild-type enzyme | Bos taurus |
Ser-1 | one additional Ser residue at the 3'-end followed by a TAA stop codon, loss of enzymatic activity above 30°C | Bos taurus |
Ser-2 | one additional Ser residue at the 3'-end followed by a TAG stop codon, loss of enzymatic activity above 30°C | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
thiosulfate + cyanide | Bos taurus | - |
sulfite + thiocyanate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
332 | - |
wild-type | Bos taurus |
353 | - |
Ser-1 | Bos taurus |
389 | - |
Ser-2 | Bos taurus |
391 | - |
one additional Arg residue at the 3'-end followed by a TAA stop codon | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
thiosulfate + cyanide | - |
Bos taurus | sulfite + thiocyanate | - |
? |