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Literature summary for 2.8.1.1 extracted from

  • Aird, B.A.; Heinrikson, R.L.; Westley, J.
    Isolation and characterization of a prokaryotic sulfurtransferase (1987), J. Biol. Chem., 262, 17327-17335.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
CN- 17°C, pH 8.5 Acinetobacter calcoaceticus
0.06
-
CN-
-
Bos taurus
3.2
-
thiosulfate 17°C, pH 8.5 Acinetobacter calcoaceticus
4
-
thiosulfate
-
Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17000
-
1 * 17000, SDS-PAGE Acinetobacter calcoaceticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
thiosulfate + cyanide Bos taurus
-
sulfite + thiocyanate
-
?
thiosulfate + cyanide Acinetobacter calcoaceticus
-
sulfite + thiocyanate
-
?

Organism

Organism UniProt Comment Textmining
Acinetobacter calcoaceticus
-
lwoffi
-
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
lwoffi Acinetobacter calcoaceticus

Reaction

Reaction Comment Organism Reaction ID
thiosulfate + cyanide = sulfite + thiocyanate + H+ double displacement mechanism Bos taurus
thiosulfate + cyanide = sulfite + thiocyanate + H+ double displacement mechanism Acinetobacter calcoaceticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
thiosulfate + 2-mercaptoethanol 20% of the activity with cyanide Acinetobacter calcoaceticus ?
-
?
thiosulfate + a thiol single displacement mechanism Acinetobacter calcoaceticus ?
-
?
thiosulfate + cyanide
-
Bos taurus sulfite + thiocyanate
-
?
thiosulfate + cyanide
-
Acinetobacter calcoaceticus sulfite + thiocyanate
-
?
thiosulfate + D,L-homocysteine 19% of the activity with cyanide Acinetobacter calcoaceticus ?
-
?
thiosulfate + dithiothreitol
-
Acinetobacter calcoaceticus ?
-
?
thiosulfate + glutathione 20% of the activity with cyanide Acinetobacter calcoaceticus ?
-
?
thiosulfate + L-cysteine 12% of the activity with cyanide Acinetobacter calcoaceticus ?
-
?
thiosulfate + N-acetyl-L-cysteine 5% of the activity with cyanide Acinetobacter calcoaceticus ?
-
?

Subunits

Subunits Comment Organism
monomer
-
Bos taurus
monomer 1 * 17000, SDS-PAGE Acinetobacter calcoaceticus