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Literature summary for 2.8.1.1 extracted from

  • Turkowsky, A.; Blotevogel, K.H.; Fischer, U.
    Properties of a soluble thiosulfate sulfur transferase (rhodanese) of the marine methanogen Methanosarcina frisia (1991), FEMS Microbiol. Lett., 81, 251-256.
No PubMed abstract available
Search for more literature for 2.8.1.1

Activating Compound

Activating Compound Comment Organism Structure
dihydrolipoate increases activity at low concentrations Methanosarcina mazei
dithioerythritol maximal activation at 0.005 mM, inhibition above Methanosarcina mazei
glutathione increases activity at low concentrations Methanosarcina mazei
L-cysteine 0.01 mM enhances activity maximally, 0.2 mM: 29% inhibition Methanosarcina mazei

Inhibitors

Inhibitors Comment Organism Structure
dithioerythritol maximal activation at 0.005 mM, inhibition above Methanosarcina mazei
DL-dihydrolipoate activation of the enzyme at 0.001 mM, inhibition above 0.0025 mM Methanosarcina mazei
glutathione activation of the enzyme with a maximum at 0.005-0.02 mM, inhibition above Methanosarcina mazei
L-cysteine 0.01 mM enhances activity maximally, 0.2 mM: 29% inhibition Methanosarcina mazei
lipoate
-
 Methanosarcina mazei
N-bromosuccinimide
-
 Methanosarcina mazei
SO32-
-
 Methanosarcina mazei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.53
-
 thiosulfate 40°C, pH 9.5 Methanosarcina mazei
1.57
-
 CN- 40°C, pH 9.5 Methanosarcina mazei

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Methanosarcina mazei
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
thiosulfate + cyanide Methanosarcina mazei
-
 sulfite + thiocyanate
-
 ?

Organism

Organism UniProt Comment Textmining
Methanosarcina mazei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methanosarcina mazei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
thiosulfate + cyanide
-
 Methanosarcina mazei sulfite + thiocyanate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Methanosarcina mazei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 20 min, stable up to, without stabilizer Methanosarcina mazei
60
-
 20 min, stable up to, thiosulfate as stabilizer Methanosarcina mazei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
11
-
-
 Methanosarcina mazei

pH Stability

pH Stability pH Stability Maximum Comment Organism
9 13 stable Methanosarcina mazei