Cloned (Comment) | Organism |
---|---|
into the vector pET28a for expression in Escherichia coli Rosetta DE3 cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
the crystal structures of selenophosphate synthetase 1 and of selenophosphate synthetase 1 complexed with AMPCP and K+ are solved to a resolution of 2.0 and 1.9 A, respectively | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | - |
Homo sapiens | |
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + selenide + H2O | Homo sapiens | - |
AMP + selenophosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P49903 | - |
- |
Purification (Comment) | Organism |
---|---|
on a Ni-chelating column, the His-tag is removed by thrombin digestion, further purification by gel-filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + selenide + H2O | - |
Homo sapiens | AMP + selenophosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
hSPS1 | - |
Homo sapiens |
selenophosphate synthetase 1 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |