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Literature summary for 2.7.9.3 extracted from
- Active bovine selenophosphate synthetase 2, not having selenocysteine (2007), Mol. Biol. Rep., 35, 541-549.
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Bos taurus |  |
| additional information | active SPS2 does not contain selenocysteine | Bos taurus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
x * 33000, SPS2, SDS-PAGE, x * 35589, SPS2 isozyme 1, sequence calculation, x * 41768, SPS2 isozyme 2, sequence calculation | Bos taurus |
| 35589 | - |
x * 33000, SPS2, SDS-PAGE, x * 35589, SPS2 isozyme 1, sequence calculation, x * 41768, SPS2 isozyme 2, sequence calculation | Bos taurus |
| 41768 | - |
x * 33000, SPS2, SDS-PAGE, x * 35589, SPS2 isozyme 1, sequence calculation, x * 41768, SPS2 isozyme 2, sequence calculation | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + selenide | Bos taurus | - |
AMP + selenophosphate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
two isozymes of SPS2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from liver, by anion exchange chromatography, gel filtration, heparin affinity and hydroxyapatite chromatography | Bos taurus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + selenide + H2O = AMP + selenophosphate + phosphate | catalytic mechanism of SPS2, overview | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + selenide | - |
Bos taurus | AMP + selenophosphate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 33000, SPS2, SDS-PAGE, x * 35589, SPS2 isozyme 1, sequence calculation, x * 41768, SPS2 isozyme 2, sequence calculation | Bos taurus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| selenophosphate synthetase | - |
Bos taurus |
| SPS | - |
Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Bos taurus | |
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Release 2023.1 (January 2023)
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