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Literature summary for 2.7.9.3 extracted from
- Functional analysis of two homologous mouse selenophosphate synthetases (2008), Biomed. Res. Trace Elem., 19, 76-79.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged SPS1 and of His-tagged SPS2 mutant Sec69Cys in Escherichia coli strain Rosetta (DE3) | Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| Sec63C | site-directed mutagenesis, SPS2 mutant Sec63Cys catalyzes the selenide-dependent synthesis of selenophosphate | Mus musculus |
| T29C | site-directed mutagenesis, the Thr29Cys mutant of SPS1 exhibits SPS activity | Mus musculus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Mus musculus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + selenide | Mus musculus | SPS2 | AMP + selenophosphate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | P97364 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged SPS1 and His-tagged SPS2 mutant Sec69Cys from Escherichia coli strain Rosetta (DE3) to homogeneity by affinity chromatography on a His-binding resin | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + selenide | SPS2 | Mus musculus | AMP + selenophosphate + phosphate | - |
? | |
| ATP + selenide | SPS2, the catalytically important selenocysteine residue is located at position 63 | Mus musculus | AMP + selenophosphate + phosphate | - |
? | |
| additional information | SPS1 shows no activity | Mus musculus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| selenophosphate synthetase 2 | - |
Mus musculus |
| Sps2 | - |
Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Mus musculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Mus musculus | |
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Release 2023.1 (January 2023)
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