Cloned (Comment) | Organism |
---|---|
expression of truncated mutant enzyme SPS-DELTAN in Escherichia coli | Aquifex aeolicus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant truncated mutant enzyme SPS-DELTAN, mixing of 0.001 ml of protein solution, containing 9.9 mg/ml protein in 20 mM Tris-HCl, pH 8.0, 2 mM dithiothreitol, and 150 mM NaCl, with 0.001 ml of a reservoir solution containing 100 mM Na HEPES, pH 7.1, 50% 2-methyl-2,4-pentanediol, 200 mM ammonium phosphate, and 50 mM ammonium sulfate and equilibrating this mixture against 0.5 ml reservoir solution at 20°C, X-ray diffraction structure determination and analysis at 2.0 A resolution, hexameric crystal structure | Aquifex aeolicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of an N-terminally truncated SPS mutant lacking 25 amino acid residues, i.e SPS-DELTAN | Aquifex aeolicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
about, analytical ultracentrifugation | Aquifex aeolicus |
68200 | - |
about, sequence calculation | Aquifex aeolicus |
204000 | - |
crystal structure | Aquifex aeolicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + selenide | Aquifex aeolicus | selenophosphate synthetase catalyzes the activation of selenide with ATP to synthesize selenophosphate, the reactive selenium donor for biosyntheses of both the 21st amino acid selenocysteine and 2-selenouridine nucleotides in tRNA anticodons | AMP + selenophosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex aeolicus | O67139 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant truncated mutant enzyme SPS-DELTAN from Escherichia coli by heat treatment at 70°C for 30 min, anion exchange and hydrophobic interaction chromatography, followed by another step of anion exchange chromatography and gel filtration | Aquifex aeolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + selenide | selenophosphate synthetase catalyzes the activation of selenide with ATP to synthesize selenophosphate, the reactive selenium donor for biosyntheses of both the 21st amino acid selenocysteine and 2-selenouridine nucleotides in tRNA anticodons | Aquifex aeolicus | AMP + selenophosphate + phosphate | - |
? | |
ATP + selenide | the phosphate moiety of selenophosphate is derived from the ATP gamma-phosphate, while the orthophosphate is from the beta-phosphate | Aquifex aeolicus | AMP + selenophosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 31000-34000, about, sequence calculation and analytical ultracentrifugation | Aquifex aeolicus |
hexamer | 6 * 31000-34000, about, crystal structure | Aquifex aeolicus |
More | SPS is a two-domain alpha/beta protein, with domain folds that are homologous to those of PurM superfamily proteins | Aquifex aeolicus |
Synonyms | Comment | Organism |
---|---|---|
selenide water dikinase | - |
Aquifex aeolicus |
selenophosphate synthetase | - |
Aquifex aeolicus |
SPS | - |
Aquifex aeolicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Aquifex aeolicus |