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Literature summary for 2.7.9.3 extracted from
- Cloning and functional characterization of an O-acetylserine(thiol)lyase-encoding gene in wild soybean (Glycine soja) (2008), Mol. Biol. Rep., 35, 527-534.
General Stability
| General Stability | Organism |
|---|---|
| labile in 0.12% sucrose monolaurate, 1% sodium cholate, and 0.2 M sodium thiocyanate, slightly stable in 2% MEGA-9, 1% sodium deoxycholate, 1% nonidet P-40 and 1% polyoxyethylene 9 lauryl ether | Bos taurus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Bos taurus | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
SDS-PAGE | Bos taurus |
| 35590 | - |
calculated from amino acid sequence | Bos taurus |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Triton X-100 | slightly stable in 1% (v/v) Triton X-100 | Bos taurus |
| urea | labile in 2 M urea | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-cellulose column chromatography, Sephacryl S-300 gel filtration, Heparin Toyopearl column chromatography, and hydroxylapatite column chromatography | Bos taurus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + selenide + H2O = AMP + selenophosphate + phosphate | bovine active SPS2 does not contain selenocysteine | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Bos taurus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, in 0.2 M Hepes buffer at pH 6.8, less than one year, stable | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + selenide + H2O | - |
Bos taurus | AMP + selenophosphate + phosphate | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Bt1 | SPS2 isoform 1 | Bos taurus |
| selenophosphate synthetase 2 | - |
Bos taurus |
| SEP synthetase | - |
Bos taurus |
| Sps2 | - |
Bos taurus |
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Release 2023.1 (January 2023)
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