Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli strain BL21 | Haemophilus influenzae |
General Stability | Organism |
---|---|
concentration-dependent inactivation | Haemophilus influenzae |
concentration-dependent inactivation | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H2O2 | 62% inhibition at 10 mM | Escherichia coli | |
H2O2 | 53% inhibition at 5 mM | Haemophilus influenzae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
selenide | pH 7.8, 37°C | Escherichia coli | |
0.025 | - |
selenide | pH 7.8, 37°C | Haemophilus influenzae | |
0.9 | - |
ATP | pH 7.8, 37°C | Escherichia coli | |
1.3 | - |
ATP | pH 7.8, 37°C | Haemophilus influenzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene selD | - |
Haemophilus influenzae | - |
gene selD | - |
Haemophilus influenzae | - |
selenocysteine containing enzyme | - |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli, to homogeneity | Haemophilus influenzae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + selenide + H2O = AMP + selenophosphate + phosphate | enzyme contains a selenocysteine residue at the active site | Haemophilus influenzae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
16 | - |
- |
Haemophilus influenzae |
29 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + selenide + H2O | - |
Haemophilus influenzae | AMP + selenophosphate + phosphate | - |
? | |
ATP + selenide + H2O | - |
Escherichia coli | AMP + selenophosphate + phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Haemophilus influenzae |
37 | - |
assay at | Escherichia coli |