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Literature summary for 2.7.9.3 extracted from
- Fetal mouse selenophosphate synthetase 2 (SPS2): Characterization of the cysteine mutant form overproduced in a baculovirus-insect cell system (1997), Proc. Natl. Acad. Sci. USA, 94, 418-421.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Spodoptera frugiperda Sf9 insect cells via baculovirus infection, expression as N-FLAG tagged protein | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Na+ | 5-20 mM Na+ has little effect on enzyme activity in the presence or absence of 1.5 mM K+ | Mus musculus |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Mus musculus | |
| Mg2+ | the optimal range for the molar ratio of Mg2+ to ATP is between 2:1 and 4:1 | Mus musculus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant from Sf9 insect cells as N-FLAG tagged protein | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + selenide + H2O | specific for ATP | Mus musculus | AMP + selenophosphate + phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mus musculus |
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Release 2023.1 (January 2023)
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