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Literature summary for 2.7.9.3 extracted from

  • Veres, Z.; Tsai, L.; Scholz, T.D.; Politino, M.; Balaban, R.S.; Stadtman, T.C
    Synthesis of 5-methylaminomethyl-2-selenouridine in tRNAs: 31P NMR studies show the labile selenium donor synthesized by the selD gene product contains selenium bonded to phosphorus (1992), Proc. Natl. Acad. Sci. USA, 89, 2975-2979.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.9.3

Inhibitors

Inhibitors Comment Organism Structure
AMP
-
 Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.046
-
 selenide pH 7.2, 37°C Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

Metals/Ions Comment Organism Structure
ammonium sulfate slightly stimulating Salmonella enterica subsp. enterica serovar Typhimurium
Mg2+ required Salmonella enterica subsp. enterica serovar Typhimurium
Mg2+ the optimal range for the molar ratio of Mg2+ to ATP is between 1:1 and 2:1 Salmonella enterica subsp. enterica serovar Typhimurium
phosphate slightly stimulating Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + selenide + H2O Salmonella enterica subsp. enterica serovar Typhimurium provides selenophosphate, that can serve as selenium donor for the reaction in which 5-methylaminomethyl-2-thiouridine is converted to 5-methylaminomethyl-2-selenouridine AMP + selenophosphate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
 wild type and mutant strain with a defective selD gene
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + dithiothreitol + H2O as effective as selenide Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
ATP + selenide + H2O selenide can be substituted by dithiothreitol, but only very poorly by L-selenocysteine and NaSH Salmonella enterica subsp. enterica serovar Typhimurium AMP + selenophosphate + phosphate
-
 ?
ATP + selenide + H2O provides selenophosphate, that can serve as selenium donor for the reaction in which 5-methylaminomethyl-2-thiouridine is converted to 5-methylaminomethyl-2-selenouridine Salmonella enterica subsp. enterica serovar Typhimurium AMP + selenophosphate + phosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium