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Literature summary for 2.7.9.3 extracted from

  • Veres, Z.; Kim, I.Y.; Scholz, T.D.; Stadtman, T.C.
    Selenophosphate synthetase. Enzyme properties and catalytic reaction (1994), J. Biol. Chem., 269, 10597-10603.
    View publication on PubMed

General Stability

General Stability Organism
3 mM H2O2, pH 7.2, in absence of DTT and EDTA, 50% loss of activity Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
8-azido-ATP competitive to ATP Escherichia coli
alpha,beta-methylene-ATP
-
Escherichia coli
AMP competitive to ATP Escherichia coli
gamma-Thio-ATP
-
Escherichia coli
Li+ in presence of K+ Escherichia coli
Na+ in presence of K+ Escherichia coli
phosphate weak Escherichia coli
Selenophosphate weak Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.9
-
ATP pH 7.2, 37°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
K+ monovalent cation required. K+, NH4+ or Rb+ Escherichia coli
Mg2+ required Escherichia coli
NH4+ monovalent cation required. K+, NH4+ or Rb+ Escherichia coli
Rb+ monovalent cation required. K+, NH4+ or Rb+ Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + selenide + H2O Escherichia coli the enzyme provides the biologically active selenium donor compound monoseleniumphosphate AMP + selenophosphate + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Storage Stability

Storage Stability Organism
-80°C, stable for 1 year Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + selenide + H2O specific for ATP Escherichia coli AMP + selenophosphate + phosphate
-
?
ATP + selenide + H2O in the absence of selenide, ATP is converted completely to AMP and phosphate Escherichia coli AMP + selenophosphate + phosphate
-
?
ATP + selenide + H2O the enzyme provides the biologically active selenium donor compound monoseleniumphosphate Escherichia coli AMP + selenophosphate + phosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
22
-
pH 5, 10 min, stable Escherichia coli
60
-
pH 7.2, aerobic conditions, 5 min, stable Escherichia coli
70
-
pH 7.2, aerobic conditions, 5 min, complete inactivation Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2 9
-
Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
22°C, 10 min, stable Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.17
-
AMP pH 7.2, 37°C Escherichia coli