General Stability | Organism |
---|---|
3 mM H2O2, pH 7.2, in absence of DTT and EDTA, 50% loss of activity | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
8-azido-ATP | competitive to ATP | Escherichia coli | |
alpha,beta-methylene-ATP | - |
Escherichia coli | |
AMP | competitive to ATP | Escherichia coli | |
gamma-Thio-ATP | - |
Escherichia coli | |
Li+ | in presence of K+ | Escherichia coli | |
Na+ | in presence of K+ | Escherichia coli | |
phosphate | weak | Escherichia coli | |
Selenophosphate | weak | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
ATP | pH 7.2, 37°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | monovalent cation required. K+, NH4+ or Rb+ | Escherichia coli | |
Mg2+ | required | Escherichia coli | |
NH4+ | monovalent cation required. K+, NH4+ or Rb+ | Escherichia coli | |
Rb+ | monovalent cation required. K+, NH4+ or Rb+ | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + selenide + H2O | Escherichia coli | the enzyme provides the biologically active selenium donor compound monoseleniumphosphate | AMP + selenophosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-80°C, stable for 1 year | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + selenide + H2O | specific for ATP | Escherichia coli | AMP + selenophosphate + phosphate | - |
? | |
ATP + selenide + H2O | in the absence of selenide, ATP is converted completely to AMP and phosphate | Escherichia coli | AMP + selenophosphate + phosphate | - |
? | |
ATP + selenide + H2O | the enzyme provides the biologically active selenium donor compound monoseleniumphosphate | Escherichia coli | AMP + selenophosphate + phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
pH 5, 10 min, stable | Escherichia coli |
60 | - |
pH 7.2, aerobic conditions, 5 min, stable | Escherichia coli |
70 | - |
pH 7.2, aerobic conditions, 5 min, complete inactivation | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | 9 | - |
Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
22°C, 10 min, stable | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
AMP | pH 7.2, 37°C | Escherichia coli |