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Literature summary for 2.7.9.2 extracted from

  • Haferkamp, P.
    Biochemical studies of enzymes involved in glycolysis of the thermoacidophilic crenarchaeon Sulfolobus solfataricus (2011), PH. D. Thesis Universität Duisburg-Essen, 2011, 0000.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.46
-
pyruvate 70°C, pH not specified in the publication Saccharolobus solfataricus
0.47
-
ATP 65°C, pH not specified in the publication Saccharolobus solfataricus
0.54
-
pyruvate 65°C, pH not specified in the publication Saccharolobus solfataricus
0.61
-
ATP 70°C, pH not specified in the publication Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
89300
-
x * 89000-92000, SDS-PAGE, x * 89300, calculated Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97V40
-
-
Saccharolobus solfataricus DSM 1617 Q97V40
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + pyruvate + H2O
-
Saccharolobus solfataricus AMP + phosphoenolpyruvate + phosphate
-
?
ATP + pyruvate + H2O
-
Saccharolobus solfataricus DSM 1617 AMP + phosphoenolpyruvate + phosphate
-
?

Subunits

Subunits Comment Organism
? x * 89000-92000, SDS-PAGE, x * 89300, calculated Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
SSO2792
-
Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.25
-
ATP 65°C, pH not specified in the publication Saccharolobus solfataricus
0.326
-
ATP 70°C, pH not specified in the publication Saccharolobus solfataricus
0.41
-
pyruvate 65°C, pH not specified in the publication Saccharolobus solfataricus
0.48
-
pyruvate 70°C, pH not specified in the publication Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.53
-
ATP 70°C, pH not specified in the publication Saccharolobus solfataricus
0.53
-
ATP 65°C, pH not specified in the publication Saccharolobus solfataricus
0.75
-
pyruvate 65°C, pH not specified in the publication Saccharolobus solfataricus
1.04
-
pyruvate 70°C, pH not specified in the publication Saccharolobus solfataricus