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Literature summary for 2.7.9.2 extracted from
- Phosphoenolypyruvate synthetase of Escherichia coli: molecular weight, subunit composition, and identification of phosphohistidine in phosphoenzyme intermediate (1977), J. Biol. Chem., 252, 3121-3127.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 77000 | - |
2 * 77000, enzyme tends to dissociate to monomers at low protein concentration | Escherichia coli |
| 150000 | - |
sedimentation equilibrium studies | Escherichia coli |
| 250000 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 255fold | Escherichia coli |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + pyruvate + H2O = AMP + phosphoenolpyruvate + phosphate | identification of phosphohistidine in phosphoenzyme intermediate | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, dephosphorylated form of enzyme, 50 mM Tris/HCl, pH 6.8, 0.2 mM EDTA, 0.2 mM DTT, 1 M sucrose, stable over a period of 12 months | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + pyruvate + H2O | - |
Escherichia coli | AMP + phosphoenolpyruvate + phosphate | - |
r |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 77000, enzyme tends to dissociate to monomers at low protein concentration | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
slightly cold-labile | Escherichia coli |
| 4 | - |
80% remaining activity after 3 days, 67% remaining activity after 1 month, pH 6.8, inactivation is reversible by prolonged incubation at 30°C | Escherichia coli |
| 30 | - |
stable at | Escherichia coli |
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