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Literature summary for 2.7.9.2 extracted from
- Phosphoenolpyruvate synthetase and pyruvate, phosphate dikinase (1974), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 10, 631-649.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| sucrose, 1.0 M, stabilizes against inactivation by heat, acidic pH, and during storage | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxoglutarate | - |
Escherichia coli |  |
| 3-phosphoglyceraldehyde | - |
Escherichia coli | |
| ADP | - |
Escherichia coli | |
| oxalacetate | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Escherichia coli | |
| additional information | - |
additional information | Km-values, both reaction directions, overview | Escherichia coli |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | divalent metal ion Mg2+ or Mn2+ required for forward reaction | Escherichia coli | |
| Mg2+ | Mg2+ is more effective | Escherichia coli | |
| Mn2+ | 4.2 to 5.6 equivalent binding sites for Mn2+ per mol of enzyme | Escherichia coli | |
| Mn2+ | divalent metal ion Mg2+ or Mn2+ required for forward reaction | Escherichia coli | |
| Mn2+ | Mg2+ is more effective | Escherichia coli | |
| additional information | enzyme contains sulfhydryl groups essential for activity | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 180000 | - |
- |
Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + pyruvate + H2O | Escherichia coli | enzyme is essential for gluconeogenesis in Escherichia coli and Salmonella typhimurium during the growth on pyruvate, lactate, alanine or serine, in certain circumstances the enzyme may also provide phosphoenolpyruvate under glycolytic conditions | AMP + phosphoenolpyruvate + phosphate | - |
r | |
| ATP + pyruvate + H2O | Escherichia coli B / ATCC 11303 | enzyme is essential for gluconeogenesis in Escherichia coli and Salmonella typhimurium during the growth on pyruvate, lactate, alanine or serine, in certain circumstances the enzyme may also provide phosphoenolpyruvate under glycolytic conditions | AMP + phosphoenolpyruvate + phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
B | - |
| Escherichia coli B / ATCC 11303 | - |
B | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + pyruvate + H2O = AMP + phosphoenolpyruvate + phosphate | mechanism | Escherichia coli | |
| ATP + pyruvate + H2O = AMP + phosphoenolpyruvate + phosphate | reaction sequence | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + pyruvate + H2O | highly specific | Escherichia coli | AMP + phosphoenolpyruvate + phosphate | - |
r | |
| ATP + pyruvate + H2O | in the reverse reaction dAMP yields 1% of the rate obtained with AMP, 3'-AMP gives no reaction | Escherichia coli | AMP + phosphoenolpyruvate + phosphate | - |
r | |
| ATP + pyruvate + H2O | enzyme is essential for gluconeogenesis in Escherichia coli and Salmonella typhimurium during the growth on pyruvate, lactate, alanine or serine, in certain circumstances the enzyme may also provide phosphoenolpyruvate under glycolytic conditions | Escherichia coli | AMP + phosphoenolpyruvate + phosphate | - |
r | |
| ATP + pyruvate + H2O | highly specific | Escherichia coli B / ATCC 11303 | AMP + phosphoenolpyruvate + phosphate | - |
r | |
| ATP + pyruvate + H2O | in the reverse reaction dAMP yields 1% of the rate obtained with AMP, 3'-AMP gives no reaction | Escherichia coli B / ATCC 11303 | AMP + phosphoenolpyruvate + phosphate | - |
r | |
| ATP + pyruvate + H2O | enzyme is essential for gluconeogenesis in Escherichia coli and Salmonella typhimurium during the growth on pyruvate, lactate, alanine or serine, in certain circumstances the enzyme may also provide phosphoenolpyruvate under glycolytic conditions | Escherichia coli B / ATCC 11303 | AMP + phosphoenolpyruvate + phosphate | - |
r | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
pyruvate formation | Escherichia coli |
| 8.4 | - |
phosphoenolpyruvate formation | Escherichia coli |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 6.8 | purified enzyme, most stable at, rapid loss of activity above pH 6.8 and below pH 5.5 | Escherichia coli |
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