Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
AMP + phosphoenolpyruvate + diphosphate | Pseudomonas fluorescens | the enzyme is dependent on AMP in the phosphotransfer networks | ATP + pyruvate + phosphate | - |
r | |
AMP + phosphoenolpyruvate + diphosphate | Pseudomonas fluorescens ATCC 13525 | the enzyme is dependent on AMP in the phosphotransfer networks | ATP + pyruvate + phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Pseudomonas fluorescens ATCC 13525 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
AMP + phosphoenolpyruvate + diphosphate | - |
Pseudomonas fluorescens | ATP + pyruvate + phosphate | - |
r | |
AMP + phosphoenolpyruvate + diphosphate | the enzyme is dependent on AMP in the phosphotransfer networks | Pseudomonas fluorescens | ATP + pyruvate + phosphate | - |
r | |
AMP + phosphoenolpyruvate + diphosphate | - |
Pseudomonas fluorescens ATCC 13525 | ATP + pyruvate + phosphate | - |
r | |
AMP + phosphoenolpyruvate + diphosphate | the enzyme is dependent on AMP in the phosphotransfer networks | Pseudomonas fluorescens ATCC 13525 | ATP + pyruvate + phosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
PPDK | - |
Pseudomonas fluorescens |
pyruvate orthophosphate dikinase | - |
Pseudomonas fluorescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
AMP | - |
Pseudomonas fluorescens | |
ATP | - |
Pseudomonas fluorescens |
Organism | Comment | Expression |
---|---|---|
Pseudomonas fluorescens | enzyme PPDK is upregulated in Pseudomonas fluorescens cells exposed to H2O2 or nitrosative stress | up |
General Information | Comment | Organism |
---|---|---|
metabolism | despite the diminished activities of enzymes involved in the TCA cycle and in the electron transport chain, the ATP levels do not appear to be significantly affected in cells stressed by the presence of hydrogen peroxide and nitrosative stress. A phospho-transfer networks mediated by acetate kinase, adenylate kinase, and nucleoside diphosphate kinase are involved in maintaining ATP homeostasis in the oxidatively challenged cells. This phospho-relay machinery orchestrated by substrate-level phosphorylation is aided by the upregulation in the activities of such enzymes like phosphoenolpyruvate carboxylase, pyruvate orthophosphate dikinase, and phosphoenolpyruvate synthase. The enhanced production of phosphoenolpyruvate and pyruvate further fuel the synthesis of ATP. The phospho-transfer system enables the organism to generate ATP irrespective of the carbon source utilized, and this metabolic reconfiguration enables the organism to fulfill its ATP need in an O2-independent manner by utilizing an intricate phospho-wire module aimed at maximizing the energy potential of PEP with the participation of AMP. While PPDK helps synthesize ATP from of phosphoenolpyruvate in the presence of AMP and diphosphate, phosphoenolpyruvate synthase produces the high energy triphosphate with the participation of AMP and phosphate. These two enzymes are markedly increased in activity in the bacteria grown in the stressed conditions. The two AMP-utilizing enzymes provide a more effective route to ATP than the traditional ADP-dependent pyruvate kinase | Pseudomonas fluorescens |
physiological function | enzyme PPDK is involved in ATP homeostasis in Pseudomonas fluorescens, overview | Pseudomonas fluorescens |