Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | [Clostridium] symbiosum |
Crystallization (Comment) | Organism |
---|---|
X-ray crystal structure mentioned, the central domain faces the C-terminal domain and positions the phosphorylated His residue for phosphoryl transfer to pyruvate thereby forming phosphoenolpyruvate and regenerating the unphosphorylated H455, native structure determined by NMR | [Clostridium] symbiosum |
General Stability | Organism |
---|---|
central domain protein Cent-I, 4.3 kcal/mol (delta G) for unfolding at 25°C in buffer at pH 7.0 | [Clostridium] symbiosum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | binds together with phosphate and ATP to the active site located at the concave surface of the N-terminal domain | [Clostridium] symbiosum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
mass spectral determination of Cent-I recombinant protein (residues 381-512 of the native PPDK), native molecular weight determined by gel filtration chromatography is 13438 | [Clostridium] symbiosum |
13438 | - |
1 * 13438, recombinant protein of the central domain of PPDK (Cent-I protein), mass spectrometry, chromatography | [Clostridium] symbiosum |
96000 | - |
x * 96000, PPDK of Clostridium symbiosum, three consecutive structural domains connected by helical linkers, X-ray crystal structure | [Clostridium] symbiosum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | [Clostridium] symbiosum | central domain transports phosphoryl-groups between two distant catalytic sites located on the N-terminal and C-terminal sites, description of ability to catalyze the ATP/phosphate partial reaction by central domain and N-terminal domain of PPDK as independent recombinant proteins | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Clostridium] symbiosum | P22983 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant proteins of the central domain (Cent-I) and of the N-terminus (Tem-340, residues 1-340 of the native PPDK) | [Clostridium] symbiosum |
Renatured (Comment) | Organism |
---|---|
percent decrease of fluorescence intensity (304 nm) for conversion of native protein (0 M urea) to fully denaturation (6 M urea) calculated (23.5%), DELTA G for unfolding of Cent-I (PPDK central domain construct of residues 381-512) in solvent determined (4.3 kcal/mol) | [Clostridium] symbiosum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + PPDK central domain construct of residues 381-512 (Cent-I) + phosphate | - |
[Clostridium] symbiosum | ? | - |
? | |
ATP + PPDK N-terminal domain construct of residues 1-340 (Tem-340) + phosphate | - |
[Clostridium] symbiosum | ? | - |
? | |
ATP + PPDK N-terminal domain construct of residues 1-553 (Tem340-Cent-I) + phosphate | - |
[Clostridium] symbiosum | ? | - |
? | |
ATP + pyruvate + phosphate | - |
[Clostridium] symbiosum | AMP + phosphoenolpyruvate + diphosphate | - |
r | |
additional information | central domain transports phosphoryl-groups between two distant catalytic sites located on the N-terminal and C-terminal sites, description of ability to catalyze the ATP/phosphate partial reaction by central domain and N-terminal domain of PPDK as independent recombinant proteins | [Clostridium] symbiosum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | x * 96000, PPDK of Clostridium symbiosum, three consecutive structural domains connected by helical linkers, X-ray crystal structure | [Clostridium] symbiosum |
monomer | 1 * 13438, recombinant protein of the central domain of PPDK (Cent-I protein), mass spectrometry, chromatography | [Clostridium] symbiosum |
More | multilayered structure of Cent-I protein in solution, chain structure of Cent-I in solution closely related to X-ray structure of native PPDK, three-dimensional structure calculated by different methods, topology of secondary structure indicated | [Clostridium] symbiosum |
Synonyms | Comment | Organism |
---|---|---|
PPDK | - |
[Clostridium] symbiosum |
pyruvate phosphate dikinase | - |
[Clostridium] symbiosum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | turnover reactions of ATP and phosphate using equal concentrations of central domain protein Cent-I (PPDK central domain construct of residues 381-512-I) and the N-terminal domain protein Tem-340 does not produce the expected products | [Clostridium] symbiosum |