Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the protein kinase/phosphatase RP catalyzes reversibly phosphorylation of T456, active form dephosphorylated at T456, diphosphate-forming dephosphorylation mechanism, assay at 25°C | Zea mays |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Zea mays |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | ADP-dependent inactivation assay of purified PPDK by bacterially-expressed RP described (47% inhibition of PPDK after 10min in presence of 2 mM ADP at 25°C), reversibility of RP activity by diphosphate-dependent reactivation assay described (1 mM diphosphate, at 25°C), SDS-PAGE and Western-Blot for detection of T456P-PPDK and RP | Zea mays | |
PPDK regulatory protein | RP catalyzes reversible phosphorylation on T456 by RP, inactive form phoshorylated at T456, ADP-dependent, a catalytic His-phophorylation precedes phosphorylation at T456, pyruvate (2 mM) can inhibit inactivation by RP | Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | P11155 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | phosphorylation/dephosphorylation by the PPDK regulatory protein RP at T456, RP-reaction described as PPDK-T456 + ADP = PPDK-T456P + AMP and PPDK-T456P + phophate = PPDK-T456 + diphosphate | Zea mays |
Purification (Comment) | Organism |
---|---|
recombinant maize PPDK used for inactivation/activation assay by the PPDK regulatory protein RP | Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Zea mays | - |
Synonyms | Comment | Organism |
---|---|---|
PPDK | - |
Zea mays |
pyruvate orthophosphate dikinase | - |
Zea mays |
pyruvate Pi dikinase | - |
Zea mays |