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Literature summary for 2.7.9.1 extracted from

  • Burnell, J.N.; Chastain, C.J.
    Cloning and expression of maize-leaf pyruvate, Pi dikinase regulatory protein gene (2006), Biochem. Biophys. Res. Commun., 345, 675-680.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information the protein kinase/phosphatase RP catalyzes reversibly phosphorylation of T456, active form dephosphorylated at T456, diphosphate-forming dephosphorylation mechanism, assay at 25°C Zea mays

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Zea mays

Inhibitors

Inhibitors Comment Organism Structure
additional information ADP-dependent inactivation assay of purified PPDK by bacterially-expressed RP described (47% inhibition of PPDK after 10min in presence of 2 mM ADP at 25°C), reversibility of RP activity by diphosphate-dependent reactivation assay described (1 mM diphosphate, at 25°C), SDS-PAGE and Western-Blot for detection of T456P-PPDK and RP Zea mays
PPDK regulatory protein RP catalyzes reversible phosphorylation on T456 by RP, inactive form phoshorylated at T456, ADP-dependent, a catalytic His-phophorylation precedes phosphorylation at T456, pyruvate (2 mM) can inhibit inactivation by RP Zea mays

Organism

Organism UniProt Comment Textmining
Zea mays P11155
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein phosphorylation/dephosphorylation by the PPDK regulatory protein RP at T456, RP-reaction described as PPDK-T456 + ADP = PPDK-T456P + AMP and PPDK-T456P + phophate = PPDK-T456 + diphosphate Zea mays

Purification (Commentary)

Purification (Comment) Organism
recombinant maize PPDK used for inactivation/activation assay by the PPDK regulatory protein RP Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Zea mays
-

Synonyms

Synonyms Comment Organism
PPDK
-
Zea mays
pyruvate orthophosphate dikinase
-
Zea mays
pyruvate Pi dikinase
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Zea mays