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Literature summary for 2.7.9.1 extracted from

  • Motti, C.A.; Bourne, D.G.; Burnell, J.N.; Doyle, J.R.; Haines, D.S.; Liptrot, C.H.; Llewellyn, L.E.; Ludke, S.; Muirhead, A.; Tapiolas, D.M.
    Screening marine fungi for inhibitors of the C4 plant enzyme pyruvate phosphate dikinase: unguinol as a potential novel herbicide candidate (2007), Appl. Environ. Microbiol., 73, 1921-1927.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
drug development the enzyme is a potential herbicide target in C4 plants Zea mays

Cloned(Commentary)

Cloned (Comment) Organism
-
Zea mays
expressed in Escherichia coli Zea mays

Inhibitors

Inhibitors Comment Organism Structure
ilimaquinone selectively toxic to C4 plants, isolated from a marine sponge, heterocyclic compound, 3 rings, 2-D structure shown Zea mays
additional information extracts from several fungal isolates selectively inhibit PPDK; for PPDK inhibition assay 5 and 10 micrograms per ml of unguinol tested in the presence of different substrate concentrations, whole-plant phytotoxicity test (C4, C3),antimicrobial assays and anti-tumor cell assays described for unguinol, 2-D structure shown for further potential candidates to inhibit PPDK that are analogues of unguinol as acarogobien A, acarogobien B and guisinol Zea mays
unguinol from fungal isolate F3000054, a mixed noncompetitive inhibitor of PPDK with respect to the substrates pyruvate and ATP and an uncompetitive inhibitor of PPDK with respect to phosphate. Unguinol has deleterious effects on a model C4 plant but no effect on a model C3 plant, effects in vivo, overview; mixed noncompetitive inhibition of PPDK with respect to the substrates pyruvate and ATP, uncompetitive with respect to phosphate, phytotoxic effects (bleaching) on C4 plants, no effect on a model C3 plant (Hordeum vulgare) Zea mays

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + pyruvate + phosphate Zea mays
-
AMP + phosphoenolpyruvate + diphosphate
-
r

Organism

Organism UniProt Comment Textmining
Zea mays
-
-
-

Purification (Commentary)

Purification (Comment) Organism
maize PPDK of leaves expressed for inhibitor studies in Escherichia coli with a His-tag and purified on a Ni-affinity column Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Zea mays
-
leaf maize PPDK of leaves used for inhibitor studies Zea mays
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + pyruvate + phosphate
-
Zea mays AMP + phosphoenolpyruvate + diphosphate
-
r
ATP + pyruvate + phosphate different substrate concentrations for PPDK inhibition assay, pyruvate (40, 80, 160, 320, 480, and 720 micromol), ATP (20, 40, 60, 120, 240, and 480 micromol), and phosphate (0.3, 0.6, 1.2, 1.8, 2.7, and 4.32 mM) Zea mays AMP + phosphoenolpyruvate + diphosphate
-
r

Synonyms

Synonyms Comment Organism
PPDK
-
Zea mays
pyruvate phoshate dikinase
-
Zea mays
pyruvate phosphate dikinase
-
Zea mays

Cofactor

Cofactor Comment Organism Structure
AMP reverse reaction Zea mays
ATP forward reaction Zea mays

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information inhibition kinetics for unguinol on PPDK Zea mays

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.04
-
-
Zea mays unguinol
0.29
-
-
Zea mays ilimaquinone