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Literature summary for 2.7.9.1 extracted from
- Glycolysis in Entamoeba histolytica. Biochemical characterization of recombinant glycolytic enzymes and flux control analysis (2005), FEBS J., 272, 1767-1783.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Entamoeba histolytica |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
AMP | pH 6.0, 37°C | Entamoeba histolytica |  |
| 0.005 | - |
AMP | pH 5.8-6.4 (optimal pH), 37°C | Entamoeba histolytica | |
| 0.02 | - |
AMP | pH 7.0, 37°C | Entamoeba histolytica | |
| 0.02 | - |
phosphoenolpyruvate | pH 5.8-6.4 (optimal pH), 37°C | Entamoeba histolytica | |
| 0.024 | - |
phosphoenolpyruvate | pH 7.0, 37°C | Entamoeba histolytica | |
| 0.03 | - |
phosphoenolpyruvate | pH 6.0, 37°C | Entamoeba histolytica | |
| 0.047 | - |
diphosphate | pH 7.0, 37°C | Entamoeba histolytica | |
| 0.068 | - |
pyruvate | pH 7.0, 37°C | Entamoeba histolytica | |
| 0.091 | - |
diphosphate | pH 6.0, 37°C | Entamoeba histolytica | |
| 0.1 | - |
diphosphate | pH 5.8-6.4 (optimal pH), 37°C | Entamoeba histolytica | |
| 0.284 | - |
ATP | pH 6.0, 37°C | Entamoeba histolytica | |
| 305 | - |
pyruvate | pH 6.0, 37°C | Entamoeba histolytica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 100000 | - |
4 * 100000, SDS-PAGE | Entamoeba histolytica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Entamoeba histolytica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Entamoeba histolytica |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50% v/v glycerol, optimal storage condition, decay factor is 3 months | Entamoeba histolytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + pyruvate + phosphate | - |
Entamoeba histolytica | AMP + phosphoenolpyruvate + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 100000, SDS-PAGE | Entamoeba histolytica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PPDK | - |
Entamoeba histolytica |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10 | - |
pyruvate | pH 6.0, 37°C | Entamoeba histolytica | |
| 15 | - |
pyruvate | pH 7.0, 37°C | Entamoeba histolytica | |
| 53 | - |
phosphoenolpyruvate | pH 7.0, 37°C | Entamoeba histolytica | |
| 80 | - |
phosphoenolpyruvate | pH 5.8-6.4 (optimal pH) | Entamoeba histolytica | |
| 87 | - |
phosphoenolpyruvate | pH 6.0, 37°C | Entamoeba histolytica | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | 6.4 | reaction with AMP, phosphoenolpyruvate and diphosphate | Entamoeba histolytica |
| 7 | - |
reaction with pyruvate, ATP and phosphate | Entamoeba histolytica |
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