Cloned (Comment) | Organism |
---|---|
- |
Entamoeba histolytica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
tetrasodium 1-hydroxyethylidene biphosphonate | - |
Entamoeba histolytica | |
tetrasodium 1-hydroxymethylidene biphosphonate | - |
Entamoeba histolytica | |
tetrasodium 1-hydroxynonane biphosphonate | - |
Entamoeba histolytica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
AMP | less than 0.005 mM ,pH 6.3, 25ºC | Entamoeba histolytica | |
0.021 | - |
phosphoenolpyruvate | pH 6.3, 25ºC | Entamoeba histolytica | |
0.1 | - |
diphosphate | pH 6.3, 25ºC | Entamoeba histolytica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | slight activation | Entamoeba histolytica | |
Mg2+ | necessary for activity. Optimal concentration of 4.5 mM | Entamoeba histolytica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
98000 | - |
x * 98000, SDS-PAGE | Entamoeba histolytica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Entamoeba histolytica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
method that includes metal affinity chromatography | Entamoeba histolytica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12 | - |
recombinant enzyme | Entamoeba histolytica |
Storage Stability | Organism |
---|---|
22ºC, Tris-HCl buffer, pH 7.9, 5 mM imidazole, 50 mM NaCl, 2 weeks | Entamoeba histolytica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + phosphate | - |
Entamoeba histolytica | AMP + phosphoenolpyruvate + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 98000, SDS-PAGE | Entamoeba histolytica |
tetramer | molecular filtration | Entamoeba histolytica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | - |
inactive below 10ºC | Entamoeba histolytica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
ATP formation | Entamoeba histolytica |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
tetrasodium 1-hydroxyethylidene biphosphonate | pH 6.3, 25ºC | Entamoeba histolytica | |
4.3 | - |
tetrasodium 1-hydroxynonane biphosphonate | pH 6.3, 25ºC | Entamoeba histolytica | |
11 | - |
tetrasodium 1-hydroxymethylidene biphosphonate | pH 6.3, 25ºC | Entamoeba histolytica |