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Literature summary for 2.7.9.1 extracted from

  • Hatch, M.D.; Slack, C.R.
    Pyruvate, Pi dikinase from leaves (1975), Methods Enzymol., 42C, 212-219.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
thiols required for activity in solution Zea mays

Inhibitors

Inhibitors Comment Organism Structure
ADP mediates a rapid but reversible inactivation in presence of a thiol Arundinaria sp.
ADP mediates a rapid but reversible inactivation in presence of a thiol Zea mays
AMP
-
Arundinaria sp.
AMP
-
Zea mays
gamma(p-Arsenophenyl)-n-butyrate
-
Arundinaria sp.
gamma(p-Arsenophenyl)-n-butyrate
-
Zea mays
p-chloromercuribenzoate
-
Arundinaria sp.
p-chloromercuribenzoate
-
Zea mays
p-hydroxymercuribenzoate
-
Zea mays
phosphate
-
Arundinaria sp.
phosphate
-
Zea mays
pyruvate
-
Arundinaria sp.
pyruvate
-
Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
AMP pH 6.8, 25ºC Arundinaria sp.
0.009
-
ATP pH 8.3, 30ºC Zea mays
0.04
-
diphosphate
-
Zea mays
0.04
-
diphosphate
-
Arundinaria sp.
0.09
-
ATP pH 8.3 Zea mays
0.11
-
pyruvate pH 8.3, 30ºC Zea mays
0.11
-
pyruvate pH 8.3, 30ºC Arundinaria sp.
0.5
-
phosphate pH 8.3, 30ºC Zea mays

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Zea mays
Mn2+ not activation Zea mays
NH4+
-
Zea mays

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
94000
-
4 * 94000, SDS-PAGE Zea mays
387000
-
sedimentation analysis Zea mays

Organism

Organism UniProt Comment Textmining
Arundinaria sp.
-
-
-
Zea mays
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, using Sephadex-G200 and Hypatite C chromatography Zea mays

Reaction

Reaction Comment Organism Reaction ID
ATP + pyruvate + phosphate = AMP + phosphoenolpyruvate + diphosphate sequential mechanism for the addition of ATP and phosphate and a ping-pong mechanism for the addition of pyruvate and release of phosphoenolpyruvate Zea mays
ATP + pyruvate + phosphate = AMP + phosphoenolpyruvate + diphosphate sequential mechanism for the addition of ATP and phosphate and a ping-pong mechanism for the addition of pyruvate and release of phosphoenolpyruvate Arundinaria sp.

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Zea mays
-
leaf
-
Arundinaria sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2
-
measured at 30ºC Zea mays

Storage Stability

Storage Stability Organism
0ºC, as a precipitate in a 66% saturated solution of (NH4)2SO4 Zea mays
0ºC, as a precipitate in a 66% saturated solution of (NH4)2SO4 Arundinaria sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + pyruvate + arsenate
-
Zea mays ?
-
ir
ATP + pyruvate + arsenate
-
Arundinaria sp. ?
-
ir
ATP + pyruvate + phosphate
-
Arundinaria sp. AMP + phosphoenolpyruvate + diphosphate
-
?
ATP + pyruvate + phosphate activity with UTP, GTP and CTP is 1-3% of the activity with ATP Zea mays AMP + phosphoenolpyruvate + diphosphate
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 94000, SDS-PAGE Zea mays

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
-
rapid inactivation, activity is recovered by re-warming to 20-30ºC Zea mays
-
-
rapid inactivation, activity is recovered by re-warming to 20-30ºC Arundinaria sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
phosphoenolpyruvate formation Arundinaria sp.